Wnt / ?-catenin Signaling Pathway In The Epithelial Mesenchymal Transition Of Adenomyosis

Objective:1.To verify the existence of epithelial mesenchymal transition(EMT)in adenomyosis.2.To study whether Wnt /?-catenin signaling pathway promotes the occurrence of epithelial mesenchymal transition(EMT)in adenomyosis.Materials and methods:1.Experimental group: 30 patients with adenomyosis confirmed by postoperative pathology were selected for hysterectomy.All patients were retained with eutopic and ectopic endometrial specimens,including 15 cases of eutopic endometrial hyperplasia and 15 cases of secretory stage.2.Control group: 30 cases of normal endometrium were taken from patients without endometrial lesions confirmed by pathology after operation(15 cases in proliferative phase and 15 cases in secretory phase).3.The expression of E-cadherin,vimentin and ?-Catenin in normal endometrium,adenomyosis eutopic endometrium and ectopic endometrium were detected by immunohistochemistry.Result:1.The expression level of E-cadherin in eutopic and ectopic endometrium of adenomyosis was lower than that in normal endometrium.There was no significant difference between eutopic and ectopic endometrium.2.The expression level of vimentin in eutopic and ectopic endometrium of adenomyosis was higher than that in normal endometrium,but there was no significant difference between eutopic andectopic endometrium.3.The expression level of ?-Catenin in eutopic and ectopic endometrium is higher than that in normal endometrium.There was no significant difference in the expression of ?-Catenin between eutopic and ectopic endometrium.4.In normal endometrium and eutopic endometrium of adenomyosis,the expression of E-cadherin,vimentin and ?-Catenin was not affected by menstrual cycle.Conclusion:1.Epithelial mesenchymal transition(EMT)is involved in the formation and development of adenomyosis.2.The activation of Wnt /?-catenin signaling pathway promotes EMT in adenomyosis.