The Expression Of RUNX1 Gene In Lung Squamous Cell Carcinoma And Its Clinical Significance

Background:Lung cancer emerges as the most common cancer malignancy worldwide and is second ranked in incidence and top ranked in mortality.Squamous-cell carcinoma of the lung is the second-most common type of lung cancer.As we enter the era of precision medicine,lung squamous cell carcinoma targeted gene mutation rate is extremely low,the treatment options for advanced squamous-cell carcinoma are very limited.Therefore,the identification of novel therapeutic targets for squamous-cell carcinoma is the focus of ensuing research.As a transcription factor,RUNX1 can suppress or promote cancer under the action of different tumor cell types and different regulatory factors.Its role and mechanism in hematological tumors have been extensively explored.This study aimed to investigate the expression of RUNX1 in lung squamous cell carcinoma and its clinical significance.Objective:The aim of this study was to observe the expression level of RUNX1 in lung squamous cell carcinoma and adjacent tissues,explore the relationship between the expression of RUNX1in lung squamous cell carcinoma and the clinical and pathological characteristics of patients and its clinical significance,and analyze the correlation between RUNX1 expression and the prognosis of patients with lung squamous cell carcinoma.Methods:This retrospective study included 113 specimens of lung squamous cell carcinoma,who were treated at Shaanxi Cancer Hospital,between January 2014 to December 2016,including 15cases of surgical lung squamous cell carcinoma tissue specimens and paired paracancerous tissue specimens with negative margins,and 98 cases of bronchoscopy biopsy and lung squamous cell carcinoma tissue specimens,using immunohistochemical technique SP method to detect the expression of RUNX1.Collecting clinical and pathological data of all patients with lung squamous cell carcinoma,and record in detail their age,gender,smoking history,whether they are combined with other tumors,tumor markers(NSE,CYFRE21-1,CA-125,CA-50,CA-199,CEA,TNF,AFP,SF),blood routine(NLR,HB,PLT),liver function(ALB,LDH),pathological staging,T staging,N staging,M staging,tumor histological classification,EGFR,ALK,multidrug-resistant associated protein(MRP,TS,Topo-Ⅱ,Pgp,LRP,GST-π,ERCC-1,Stathmin,TUBB-3).All patients were retrieved,reviewed and registered with electronic medical records,and followed up by telephone.The follow-up date ended on January 21,2021.27 cases were lost to follow-up,accounting for 23.9%,66 cases died,accounting for 58.4%,and 20 cases survived,ccounted for 17.7%.Using SPSS26.0 statistical software to analyzing the data results.The expression of RUNX1 in lung squamous cell carcinoma tissues and its paired adjacent tissues was compared using a two-tailed t-test.The correlation between the expression of RUNX1 in tissues and its clinical and pathological parameters was tested by chi-square.Using Kaplan-Meier survival analysis method,log-rank statistical test were used for prognostic analysis.The Cox proportional hazard model was used for multivariate survival analysis.P<0.05 was considered statistically significant.Results:1.The expression of RUNX1 in lung squamous cell carcinoma tissues was significantly higher than that of paired adjacent tissues(t=2.824,P﹤0.05).2.The expression of RUNX1 in lung squamous cell carcinoma tissue was negatively correlated with T stage(χ~2=7.126,P<0.05),N stage(χ~2=5.628,P<0.05),M staging(χ~2=12.834,P<0.05)and pathological stage(χ~2=4.575,P<0.05).3.The overall survival time of patients with high expression of RUNX1 was longer than that of patients with low expression(P<0.0001).4.Loss of RUNX1 is an independent risk factor for the prognosis of lung squamous cell carcinoma(HR=0.359,P=0.002).Conclusion:RUNX1 is involved in the occurrence of early lung squamous cell carcinoma but may be a tumor suppressor to inhibit the development of lung squamous cell carcinoma.The absence of RUNX1 is one of the independent risk factors for the prognosis of lung squamous cell carcinoma.