| Objective:To analyze the fecal culture and urine culture of patients with primary biliary cholangitis(PBC),and to study the effects of intestinal flora imbalance and urinary tract bacterial infection on the immune function of PBC,in order to find a new treatment scheme for adjuvant treatment of PBC.Methods:Fifty-eight PBC patients and 58 healthy people in the normal control group were selected,the urine and feces culture results and immune function of PBC and control group were collected,PBC was divided into early stage and progressive stage,to observe the relationship between disease progression and PBC bacterial infection.And then according to the results of urine and feces culture to analyze the effect of bacterial infection on immune function in PBC.Results:1.There was no significant difference in age and sex between PBC group and control group(P > 0.05);The positive rates of urine culture and feces culture in the PBC group were 43.1% and 22.4%,while those in the control group were 6.9% and 3.4%,and the difference between the two groups was statistically significant(P < 0.05).2.The positive rate of urine culture was 33.3% in the early stage of PBC and 51.6%in the progressive stage.The positive rate of feces culture was 11.1% in the early stage of PBC and 32.3% in the progressive stage.With the progression of PBC disease,the positive rate of urine and feces culture was slightly higher than that in the early stage,but the difference between the two stage was not statistically significant(P > 0.05).3.The comparison of immunological data between the early stage of PBC and the progressive stage showed that the total T lymphocyte level in the progressive stage was significantly lower than that in the early stage of PBC,and the difference was statistically significant(P < 0.05).The other items included IL-2、IL-6、IL-10、IL-17、TNF-a、IFN-r、Th1、Th17、Th2、Treg、Th1/Th2、Th1/Treg、Th17/Treg、total B、Th、NK cell,and there were no statistical significance between the two groups(P > 0.05).4.The comparison of immunological data between the PBC group and control group that the IFN-γ,IL-2,IL-6,IL-10,TNF-a,IL-17,Th1,Th17,Th1/Th2,Th17/Treg,Th1/Treg level of PBC were significantly higher than those in control group,and the other items included Th2,Treg,tatol T,NK cell of PBC significance lower than that in the control group,and there were statistical significance between the two groups(P <0.05);there were no statistically significant differences in TH cell and total B lymphocytes(P>0.05).5.The level of IFN-γ,TNF-a,Th1,Th1/Th2,Th1/Treg in the positive was significantly higher than that in the negative of urine culture,and the differences were statistically significant(P<0.05),there was no statistically significant difference in IL-2,IL-6,IL-10,IL-17,Total T,NK,TH,total B cells,Th2,Th17,Treg,Th17/Treg(P>0.05).6.The level of IFN-γ,Th1/Th2 in the positive was significantly higher than those in the negative of feces culture,and the differences were statistically significant(P<0.05),there were no statistically significant different in IL-2,IL-6,IL-10,TNF-a,IL-17,total T,NK,TH,total B,Th1,Th2,Th17,Treg,Th17/Treg,Th1/Treg(P>0.05).7.The Spearman rank correlation analysis showed that Escherichia coli,the main pathogenic bacteria of urinary tract infection,was positively correlated with IFN-γ and TNF-a levels(r>0,P<0.05),there was no significant correlation with Th1,Th17,Th2,Treg,Th1/Th2,Th17/Treg,Th1/Treg,IL-2,IL-6,IL-10,IL-17,total T,NK,Th,total B lymphocytes(P > 0.05).Conclusion:1.Some PBC patients have intestinal flora imbalance and urinary tract infection incidence significantly higher than normal people,which may be related to the occurrence and development of PBC;The higher rate of urinary tract infection among women may be one of the reasons for the female predominance in PBC.2.Bacterial infection can stimulate the increased secretion of pro-inflammatory cytokines(Th1,IFN-γ,IL-17,TNF-a.)in PBC,further aggravating the immune dysfunction of PBC,which may be related to the poor prognosis of PBC. |
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