Transcriptome Analysis Of Brain Cortex In BTBR Mouse Model Of Autism Spectrum Disorder

Objective: In this study,we carried out a transcriptome analysis of brain cortex in BTBR mouse model of autism spectrum disorder(ASD),using RNA-sequencing(RNA-seq).Thus reveal some possible pathological mechanisms of autism spectrum disorder,and preliminarily explore some biomarkers for ASD diagnosis and provide drug intervention targets for ASD.Methods: 1.The cerebral cortex tissue of BTBR mice and C57BL/6J(B6)mice were separated respectively.We extract total RNA from cerebral cortex tissue,and enrich the m RNA by magnetic beads,and then construct c DNA library by reverse transcription,and sequence using Illumina Hi Seq4000 sequencer.2.We filter the sequencing results to obtain the final data,and refer the genome comparison to obtain the gene expression results.After comparing the data of the BTBR mice group and the B6 mice group,we screen differentially expressed genes(DEGs),and perform GO function enrichment analysis on them.3.We select 4 DEGs related to ASD or brain development,and verify the selected DEGs using real-time quantitative PCR method.Results: 1.The c DNA library was successfully constructed and sequenced.We filtered the sequencing results,and the results showed that the sequencing data was high and could be analyzed.2.A total of 1271 DEGs were discovered between BTBR mice and B6 mice.Among which 575 DEGs were up-regulated while 696 DEGs were down-regulated in BTBR mice when compared to the B6 mice.GO function enrichment analysis displayed DEGs were obviously enriched in muscle filament sliding,immune response and regulation of activation of membrane attack complex.3.Real-time fluorescence quantitative PCR results showed that the relative expression of the four selected DEGs was consistent with the results which were obtained in RNA-seq.Conclusion: 1.This study successfully conducted transcriptomics analysis on the cerebral cortex of the BTBR mouse model of autism spectrum disorder,using RNA-seq technology and bioinformatics.After verification by real-time fluorescent quantitative PCR experiments,it was identified that the sequencing results were acceptable.2.This study found that there were 1271 DEGs in the cerebral cortex of BTBR mice and B6 mice,some of which are clearly related to ASD and brain development.Based on these genes and upstream and downstream regulatory factors,it can provide new directions for the search for diagnostic markers for ASD,and provide some possible drug targets for the treatment of ASD.