Effect Of Sodium Aescinate On Liver Fibrosis Induced By Bile Duct Ligation

Objective This research group explored the effect of sodium aescinate on liver fibrosis induced by bile duct ligation in rats and further studied the molecular mechanism of its influence on liver fibrosis to provide new ideas and methods for the treatment of obstructive jaundice in clinical workMethods 54 male SD rats were randomly divided into: the control group(Sham)which were only given equivalent normal saline(NS)injection each day after laparotomy,objective jaundice group(OJ group)which were only given equivalent normal saline(NS)injection each day after bile duct ligation(BDL),sodium aescinate group(SA)which were given sodium aescinate(SA)injection at the dose of 3.6 mg·kg-1each day after bile duct ligation.Observing the change of morphological structure and the collagen fiber of rats’ liver through HE and Masson staining.Detecting ALT、AST、TBIL through ELISA.Detecting collagen I and collagen III through immunofluorescence.Detecting P-4ebp1、eIF-4E、4ebp1through immunohistochemical and Western-blot.Results In OJ group,the liver structure was disordered,serum levels of ALT,AST and TBIL were significantly increased,collagen I and collagen III,were significantly increased,and the expressions of EIF-4E and P-4EBP1 in liver cells were decreased.The serum levels of ALT,AST and TBIL in SA-treated rats were significantly lower than those in OJ group..SA can down-regulate the expression of collagen I and collagen III in the liver of BDL rats.The expression levels of eif-4E、P-4ebp1、4ebp1 in liver cell of BDL rats were increased significantlyConclusions Sodium aescinate protects hepatocytes by up-regulating the expression of p-4EBP1 and eIF-4E,thereby slow down the hepatic fibrosis.