| Objective:The expression of DGCR8 in laryngeal squamous carcinoma and para-carcinoma tissue was studied so as to analyze whether there were differences and provide a new outcome indicator for laryngeal cancer.Besides,the correlation of DGCR8and laryngeal squamous carcinoma patients’clinical information,pathological characteristics and survival prognosis was explored to discuss the relationship between DGCR8 and incidence and progress of laryngeal squamous cell carcinomas,and then assess the feasibility of using DGCR8 as the further development and outcome indicator of laryngeal squamous carcinoma.Methods:(1)A total of 25 cases of patients who were treated at our hospital from December 2019 to December 2020 and were clinically and pathologically diagnosed with laryngeal squamous cell carcinomas were selected as the research objects.Their clinical pathologic information was recorded.The expression of DGCR8m RNA and DGCR8 in laryngeal squamous carcinoma and para-carcinoma tissue was detected using the q RT-PCR and Western blot(WB)technology.The correlation between DGCR8m RNA and the patients’gender,age,T stage,clinical stage,lymph node metastasis and pathological grading of LSCC patients was explored throughχ~2.(2)123 cases of laryngeal squamous cell carcinoma samples and para-carcinoma samples in TCGA database were analyzed using bioinformatics technology.In addition,the data were analyzed through limma R bags and perl language.The difference of the DGCR8 expression amount in the laryngeal squamous cell carcinoma samples and para-carcinoma samples was explored through Wilcoxon test.R software Wilcoxon and Kruskal Wallis test was used to analyze the relationship between DGCR8 expression amount in laryngeal squamous carcinoma with patients’age,sex,tumor stage,lymphatic metastasis and pathological grading.As for the combined survival information data after being treated with Kaplan-Meier method,the relationship between DGCR8 expression and survival prognosis of laryngeal squamous cell carcinoma was analyzed using R software package.Results:(1)According to PCR experiment results,the expression of DGCR8m RNA in laryngeal squamous cell carcinoma tissues was significantly higher than that in para-carcinoma tissues(P<0.01);the expression of DGCR8m RNA was different in T stage(P<0.05);the expression of DGCR8m RNA showed no difference in different genders,ages,lymph node metastasis,and clinical stages(P>0.05).(2)WB experiment results showed that the expression of DGCR8 protein in laryngeal squamous cell carcinoma was significantly higher than that in para-carcinoma tissue(P<0.01).(3)As seen from the bioinformatics results,the expression of DGCR8 in laryngeal squamous carcinoma samples was significantly higher than in the para-carcinoma tissue samples(P<0.05);the expression of DGCR8 in T1+T2 stage was higher than that in T3+T4 stage(P<0.05);there was no statistical significance for the expression of DGCR8 in terms of gender,age,lymph node metastasis,clinical stage and pathological grading(P>0.05);DGCR8 had no significant correlation with survival prognosis of laryngeal squamous cell carcinoma(P>0.05).Conclusion:1.The expression of DGCR8 in laryngeal squamous cell carcinoma tissues is significantly higher than that in para-carcinoma tissues,and the expression of DGCR8 is correlated with T staging of laryngeal squamous cell carcinoma,suggesting that DGCR8 is related to the occurrence and development of laryngeal squamous cell carcinoma.2.The results of bioinformatics analysis are similar to the results of the experiment.The expression of DGCR8 in laryngeal squamous cell carcinoma is higher in T1+T2 stage than in T3+T4 stage,suggesting that it is expected to be an indicator of early screening index for laryngeal squamous cell carcinoma.The trend of the line graph indicates that the5-year survival rate of patients with high expression of DGCR8 is higher,but there is no statistically significant difference,suggesting that the expression of DGCR8 is not significantly correlated with the prognosis of laryngeal squamous cell carcinoma. |
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