The Effects Of Histone H3K9me Methylation Modification Imbalance On Wheezing Diseases In Children

Objective:To investigate the effects of histone H3K9 me methylation modification imbalance on wheezeing diseases in children,and provide a new theoretical basis for the clinical prevention and treatment of children’s wheezing diseases.Methods:From November 2018 to June 2020,children who were admitted to the Department of Pediatrics of the Affiliated Hospital of Zunyi Medical University with indications for bronchoscopy severe pneumonia with breathing,severe pneumonia with breathing(recovery),severe pneumonia without breathing,bronchial foreign body,congenital laryngeal cartilage dysplasia,airway deformity children(239 cases)in bronchoalveolar lavage fluid(Broncho Alveolar lavage fluid(BALF)was divided into control group,wheezing group(acute phase group),non-wheezing group and wheezing recovery group..Perform the following tests: Micro-sample multi-index flow protein quantification technology(Cytometric Bead Array,CBA)to detect the level of the cytokines Interferon-γ(Interferon-gamma,IFN-γ)、Interleukin-6(Interleukin-6,IL-6)、Interleukin-1(Interleukin-1,IL-1)、Interleukin-10(Interleukin-10,IL-10)in BALF;Western blot(WB)detection of BALF The methylation level of histones H3K9me2 and H3K9me3;immunofluorescence was used to detect the methylation level of histone H3K9me2 and the expression level of histone methylase G9α in BALF.Results:(1)The CBA results showed that the expression of IL-1 and IL-6 in BALF in the wheezing group were significantly higher than those in the non-wheezing group and the control group(P ﹤0.05),and the expression level of IL-10 in the wheezing group was higher than that in the non-wheezing group And the control group was significantly reduced(P ﹤ 0.05),while the expression level of IFN-γ did not change significantly between the groups of children(P > 0.05).(2)WB results showed that compared with the non-wheezing group and the control group,the methylation levels of histones H3K9me2 and H3K9me3 in the wheezing group were significantly increased(P﹤0.01).(3)The immunofluorescence staining results showed that compared with the non-wheezing group and the control group,the wheezing group histone H3K9me2 methylation level and histone methylase G9α expression level were significantly increased(P﹤0.05),but not wheezing Compared with the control group,the methylation level of H3K9me2 and the expression level of histone methylase G9α did not change significantly(P > 0.05).(4)WB results showed that compared with severe pneumonia with wheezing(acute phase),severe pneumonia with wheezing(recovery phase)H3K9me2 and H3K9me3 methylation levels were significantly lower(P﹤0.05).Conclusion:(1)The abnormal expression of cytokines IL-1,IL-6 and IL-10 in BALF may be involved in the pathogenesis of childhood wheezing diseases.(2)The imbalance of histone H3K9me2 and H3K9me3 methylation modification may be involved in the occurrence and development of childhood wheezing diseases.