| Objective:To investigate the expression of long noncoding RNA HOTAIRM1 in cervical cancer and its effect on the proliferation of cervical cancer cells.Methods: Thirty pairs of cervical cancer tissue samples and paired non-tumor cervical tissue samples were collected from Jiangxi Maternal and Child Health Hospital from January 2017 to January 2019,and four cervical cancer cells(C33A,Hela,caski,siha)and normal cervical squamous epithelial cells H8 were selected.The expression level of LncRNA HOTAIRM1 in 30 pairs of tumor and tissues/adjacent tissues and cervical cancer cell lines C33 A,Hela,caski,siha and normal cervical squamous cell line H8 was detected by q RT-PCR.CCK8 test and flow cytology test were used to detect the effect of down-regulating the expression of LncRNA HOTAIRM1 on the proliferation and cycle of Hela cells.In WB test,si RNA silenced LncRNA HOTAIRM1 and then used CCK8 to detect cell proliferation,flow cytology test to detect cell cycle and western blot to detect the change of cyclin expression.According to bioinformatics analysis,miRNA-137 is the target gene of LncRNA HOTAIRM1,and the regulation of LncRNA HOTAIRM1 on miRNA-137 is verified by experiments such as luciferase reporter gene.Results: Compared with the adjacent tissue of cervical cancer,the expression of HOTAIRM1 in cervical cancer tissues was significantly increased;the expression of HOTAIRM1 in Hela cells was significantly increased compared with that in normal cervical cells H8;knocking down HOTAIRM1 in Hela cells could significantly inhibit the proliferation of Hela cells;q RT-PCR and luciferase reporter gene were used.Experiments confirmed that LncRNA HOTAIRM1 adsorbed micro RNA-137,thus affecting the proliferation of cervical cancer cells.Conclusions: LncRNA HOTAIRM1 can promote the proliferation of cervical cancer cells,and its mechanism may be related to the adsorption of micro RNA-137,which will provide a target for the treatment of cervical cancer. |
PDF Full Text Request