Difference Analysis Of Vital Organs Infection By Mouse Cytomegalovirus In Nude Mice And Meta-analysis Of Ganciclovir Combined Gammaglobulin In The Treatment Of Infant Cytomegalovirus Hepatitis

PartⅠ Difference analysis of vital organs infected by mouse cytomegalovirus in nude mice Experimental ObjectiveAfter murine cytomegalovirus(MCMV)infection in important organs of nude mice,the viral load of different organs and the pathological characteristics of the damage were analyzed to provide a basis for clinical work and future research on human cytomegalovirus(HCMV)infection in children with immunodeficiency.Experimental MethodsNude mice were divided into two groups,A and B,each in group A was intraperitoneally injected with 0.25 m L(5×103 PFU)MCMV,and each in group B was intraperitoneally injected with 0.25 m L cell maintenance solution.The mice were fed in isolation and observed for 7 days before death,and their hearts,liver,spleen,lungs and kidneys were extracted strictly aseptically.One was used to extract MCMV-DNA for fluorescence quantitative PCR detection;One was fixed with 10% paraformaldehyde and embedded in paraffin to pathology.Experimental ResultsFluorescence quantitative PCR results showed that MCMV-DNA was expressed in the heart,liver,spleen,lung and kidney of nude mice 7 days after MCMV infection,suggesting that these important organs were all infected with MCMV.The expression of MCMV-DNA was the highest in lung,followed by heart,kidney,spleen and liver.The results of pathological sections showed that the liver and spleen tissues had undergone morphological changes,and there were visible viral nuclear inclusions and characteristic changes in the eye signs of the "owl" in the liver and spleen,and inflammatory cells and inflammatory cells were visible in the interstitial tissue;No morphological changes were observed in the lung,heart and kidney of other organs,only a few inflammatory cells were observed in the interstitium.Experimental conclusionAfter intraperitoneal injection of the experimental dose and virulence of MCMV to nude mice for 7d,liver cells and spleen cells may be more vulnerable to damage after MCMV infection.In the future,this study will expand the research scope to detect the expression level and pathological evidence of MCMV-DNA in the heart,liver,spleen,lung,kidney,brain tissue and retina at more time points,so as to obtain more sufficient data.PartⅡ Analysis of clinical efficacy of ganciclovir combined with gammaglobulin in the treatment of infant cytomegalovirus hepatitisExperimental Objective To compare the clinical efficacy of ganciclovir combined with gamma globulin and ganciclovir in the treatment of infantile cytomegalovirus hepatitis through a systematic evaluation.Experimental Methods Search the CNKI,VIP,Pub Med and Wanfang databases by computer.The search time is from the establishment of each database to June 30,2020.In the search,basic liver protection,jaundice,etc.and or ganciclovir treatment alone were used as the control group,and gamma globulin treatment was added to the control group as the experimental group.The quality of the included literatures were evaluated by the Jadad scale.Rev Man5.4 statistical software was used for data analysis.Experimental Result The quality evaluation of the included literatures were low quality.A total of 9 articles were included,with 626 patients,including 327 in the treatment group and 299 in the control group.Meta analysis results show: 1.TBIL level: MD=-12.58,95%CI(-15.90,-9.25),P < 0.00001;DBIL level:MD=-14.36,95%CI(-19.28,-9.43),P <0.00001;ALT level: MD=-25.70,95%CI(-41.86,-9.54),P=0.002.2.Total effective rate: OR=5.93,95%CI(3.36,10.47),P < 0.00001;Negative conversion rate of blood CMV-IGM: OR=7.08,95%CI(1.74,28.80),P=0.006;CMV-DNA negative conversion rate in blood/urine:OR=7.08,95%CI(1.74,28.80),P=0.006.Experimental Conclusion In terms of the laboratory indicators of improving cytomegalovirus infected hepatitis in infants,ganciclovir combined with gamma globulin was significantly better than ganciclovir alone,and the incidence of adverse reactions of ganciclovir combined with gamma globulin in the statistical literature was generally lower than ganciclovir alone.However,considering that all the included literatures were of low quality,higher quality literatures are needed to confirm the results.In addition,the use of standardized testing or self-reporting of adverse events is also needed to better understand the factors involved.