| Purpose:The main producing areas of Pseudostellaria Radix are Zherong,Fujian and Shibing,Guizhou.The main drying methods are sun-dried or dried.A unified and scientific production area processing specification has not yet been formed.The randomness and blindness of the drying process have led to the quality of Pseudostellaria Radix.Different,the optimization of its drying method urgently needs further research.This topic will use the fingerprints of Pseudostellaria Radix and its hypoglycemic effect to establish the spectrum-effect relationship,combine the determination of drying efficiency,appearance,polysaccharide and extract content,etc.,to comprehensively evaluate the quality of the pieces of Pseudostellaria Radix with different drying processes.It is the best method for the drying process of Pseudostellaria Radix.Make sure to provide the basis for the drying process of decoction pieces.Methods:1.Use three methods of sun drying,shade drying,and drying at different temperatures to dry different specifications of fresh Pseudostellaria Radix.The drying rate,appearance properties,polysaccharide and extract content are used as evaluation indicators to initially determine the best drying of Pseudostellaria Radix.2.Measure the fingerprints of Pseudostellaria Radix decoction pieces with different drying processes in the general election,and perform fingerprint similarity,clustering and principal component analysis.3.Induce the mouse diabetes model by streptozotocin,give different drying process Pseudostellaria Radix decoction to compare the efficacy of different drying processes;use the grey relational statistical method to study the spectrum effect relationship.Results:1.Staged variable temperature drying is the best drying method,followed by sun drying.The best stepwise variable temperature drying process parameters for different specifications of Pseudostellaria Radix are different.When Pseudostellaria Radix is selected for general election,the best drying process parameters are 50°C6 h-60°C12 h,and the sample load is 7.5 kg/m~2;For the selection,the best drying process parameters are 50°C6 h-60°C8 h,and the sample load is 5 kg/m~2;when Pseudostellaria Radix is a small selection,the best drying process parameters are 45°C6 h-55°C 4 h,the sample load is 5 kg/m~2.2.The measurement specifications are the fingerprints of different drying processes of Pseudostellaria Radix.There are 11 common characteristic peaks.Through cluster analysis,the samples of Pseudostellaria Radix with different drying processes are mainly divided into 2 In the principal component analysis,the cumulative contribution rate of the first four principal components reached86.070%.Peaks 1,2,3,5,8,and 11 are highly correlated with the first and second principal components,peak 7 is highly correlated with the third principal component.3.Compared with the model group,the polysaccharides and decoction of Pseudostellariae Radix can significantly increase the weight of mice(P<0.01),and the polysaccharide group>the temperature-changing drying decoction group>the sun-dried decoction group>the constant temperature decoction Group;and can significantly reduce the blood sugar level of diabetic mice(P<0.01),and the polysaccharide group>stage variable temperature drying water decoction group>sun-dried water decoction group>constant temperature water decoction group;The glucose tolerance of diabetic mice was improved(P<0.01),and the polysaccharide group>the temperature-changing drying water decoction group>sun-dried water decoction group>the constant temperature water decoction group.In summary,both the polysaccharides and decoction of Pseudostellariae Radix have a hypoglycemic effect,and the hypoglycemic effect of the decoction of the stepwise temperature-changing drying process is better than that of the sun-dried process.The gray correlation statistics method was used to correlate the common peak area in the HPLC fingerprint of the decoctions of sun-dried/constant temperature drying/segmental variable temperature drying process with the antidiabetic efficacy,and to study the correlation of the spectrum effect.According to the degree of correlation,it is determined that the compounds represented by the seven peaks 1,2,3,etc.in the fingerprint of Pseudostellariae Radix contribute to the hypoglycemic effect;it is more consistent with the main components of the fingerprint selection.Therefore,the best selection of Pseudostellariae Radix decoction pieces The best drying process parameters for the general election are 50°C 6 h-60°C 12 h,and the sample load is 7.5 kg/m~2.Conclusion:1.Three methods of sun drying,shade drying,and drying at different temperatures are used to dry different specifications of Pseudostellariae Radix.The drying rate,appearance,content of polysaccharides and water-soluble extracts are used as the inspection indicators to preliminarily determine Pseudostellariae Radix.The best drying process is staged variable temperature drying.2.The fingerprints of Pseudostellariae Radix decoction pieces with different drying processes of the general selection,with a total of 11 common characteristic peaks.Through cluster analysis,Pseudostellariae Radix samples with different drying processes are mainly divided into 2 groups;in principal component analysis,the first 4 The cumulative contribution rate of the principal components reached 86.070%,and peaks 1,2,3,5,8,and 11are highly correlated with the first and second principal components,peak 7 is highly correlated with the third principal component,These 7 peaks The chemical composition represented may play an important role in determining the quality difference of Pseudostellaria Radix with different drying processes.3.The polysaccharides and decoction of Pseudostellariae Radix have a strong hypoglycemic effect.The samples with the highest hypoglycemic activity in the decoction of Pseudostellariae Radix are the step-variable temperature drying process samples.According to the correlation degree,it is determined that the compounds represented by the peaks in the fingerprint of Pseudostellariae Radix,such as peaks 1,2,and 3,contribute to the hypoglycemic effect,which is consistent with the principal component analysis results of the fingerprint.Therefore,the best drying process parameters for the general election are 50°C 6 h-60°C 12 h,and the sample load is 7.5 kg/m~2. |
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