Analysis Of Pathogen Distribution And Drug Resistance In NICU Of A Grade 3 And First-class Hospital In Enshi

Objective Children with positive bacterial culture and various specimens in the NICU of a Grade A hospital in Enshi area from January 2017 to December 2019 were collected to analyze the characteristics of infected children,the distribution of common pathogenic bacteria and the resistance of pathogenic bacteria,so as to provide medical basis for the management of clinical neonates and the reasonable selection of antibiotics.Methods By collecting all kinds of samples of pathogenic bacteria,including blood,sputum,cerebrospinal fluid,and various secretions,from children admitted in the NICU of the Affiliated Hospital of Hubei University for Nationalities from January 2017 to December 2019,the composition,distribution and drug resis-tance of the main pathogenic bacteria were analyzed.Results1.(1)From January 2017 to December 2019,a total of 5645 samples were submitted to the NICU of children hospitalized in a Grade A hospital in Enshi.A total of 327 strains of pathogenic bacteria were isolated from the samples submitted for examination,with a positive culture rate of 5.79%.There were no differences in gender,birth weight and seasonal distribution among the three years(P>0.05),which were correlated with gestational age.The number of pathogenic bacteria detected in preterm infants in 2019 was significantly increased(P<0.05).(2)Gram-negative bact-eria were the main pathogens of NICU in our hospital.From 2017 to 2019,the num-ber and propor-tion of Gram-negative bacteria detected increased year by year(P<0.05).The com-mon Gram-positive strains were Staphylococcus aureus,Staphylococcus epidermidis and Staphylococcus hemolyticus.Gram-negative strains were Escherichia coli,Klebsiella pneumoniae and Pseudomonas aeruginosa.The fungi were Candi-da near-smoothing,Candida albicans and Candida dublinensis.(3)Pathogenic bacteria specimen composition: sputum culture took the first place,followed by secretions culture and blood culture.(4)The detection rate of Gram-negative bacteria and fungi in the preterm infant group was higher than that in the term infant group,while the detection rate of Gram-positive bacteria in the preterm infant group was lower than that in the term infant group.(5)Gram negative bacteria is the main pathogenic bacteria in our hospital NICU detection,NICU in our hospital wards common three kinds of gram-negative bacteria susceptibility results analysis can get e.coli to ampicillin resistant rate 81.1%,at the same time to the cephalosporin class of antibiotics resistance at higher levels,klebsiella pneumoniae of chloramphenicol,amikacin,levofloxacin,minocycline,beauty e.faecalis percentages of all below 10%;Pseudomonas aeruginosa is sensitive to most antibiotics.Three Gram-positive strains,vancomycin,teicolanin and linezo-lid,were highly sensitive.Conclusion1.The distribution of pathogenic bacteria in the NICU ward of our hospital was still dominated by Gram-negative bacteria,and the number and corresponding proportion of the detected strains increased year by year,followed by Gram-positive bacteria and fungi.2.The gram-negative bacteria in e.coli,klebsiella pneumoniae,pseudomonas aeruginosa,as the main pathogenic bacteria,e.coli,klebsiella pneumoniae half-and-half synthetic penicillin and cephalosporin antibiotic resistance is higher,most resistant rate over 60%,to piperacillin he azole,cefoperazone shu ba jotham,minocycline,beauty e.faecalis sensitivity is better.Pseudomonas aeruginosa is sensitive to most antibiotics,and the drug resistance rate is less than 10%.Staphylococcus aureus,Staphylococcus epidermidis and Staphylococcus hemolyticus were the main gram-positive bacteria detected,with high drug resistance rate to penicillin and azithromycin,and high sensitivity rate to vancomycin,teicolanin and linezolid.