The Relationship Between The Function Of Dcs And The HBVCCCDNA Load In Peripheral Blood Mononuclear Cells Of Patients With Chronic Hepatitis B Virus Infection

Objective: To investigate the HBVcccDNA load in PBMCs and DCs from patients with CHB; and to analyze the relationship between the maturity and function of DCs with the HBVcccDNA load in PBMCs or DCs form the patients with CHB.Methods: peripheral blood samples were collected from twenty nine patients with chronic hepatitis B and ten healthy controls. PBMCs were isolated freshly and induced with the cytokines of GM-CSF and IL-4, and a large amount of DCs were harvested after culture for 7 days. The expression of CD209, CD80, CD86, HLA-DR and CD1a of DCs was analyzed by flow cytometry. The HBVcccDNA load in PBMCs and DCs were detected by PCR. The level of IL-12 in culture supernatant of DCs was detected by ELISA. The effects on T lymphocyte proliferation induced by DCs were tested by MLR. Results:①The HBVcccDNA could be detected in PBMCs in 16 patients while could not be detected in DCs in all of 29 patients.②The HBVcccDNA load was significantly negative with the expression of the CD209, CD80, CD86, CD1a and HLA-DR on DCs of HBVcccDNA positive patients, as same as the level of IL-12 and the effects on allogenic T lymphocyte proliferation induced by DCs (CD209: r=-0.793,P<0.001;CD80: r=-0.581,P<0.05;CD86:r=-0.698,P<0.01;HLA-DR:r=-0.817,P<0.001;CD1a:r=-0.734,P<0.01;IL-12:r=-0.632,P<0.05;CPM:r=-0.617,P<0.05).③The expression of CD209, CD80, CD86, CD1a and HLA-DR, the level of IL-12 in culture supernatant of DCs and the effects on allogenit T lymphocyte proliferation induced by DCs in PBMCs HBVcccDNA positive patients were significantly lower than that in healthy people. In addition,the decreasing extent of the parameters mentioned above in PBMCs HBVcccDNA positive patients was greater than in PBMCs HBVcccDNA negative patients(CD209 : 28.79±3.91VS 35.39±5.11;CD80: 18.79±2.82 VS 33.42±8.73;CD86: 18.27±3.82VS 35.13±8.25;HLA-DR : 26.81±4.57 VS 49.21±7.85; CD1a:27.43±4.71VS 48.98±4.89;IL-12:20.01±5.32VS35.22±7.38; CPM:116.01±39.65VS 273.15±92.62。P<0.05 or P<0.001).Conclusion: The function and maturity of DCs in patients with CHB was worse than that of healthy controls; HBVcccDNA was detected in PBMCs from patients with CHB; moreover, the higher the load of HBVcccDNA is in PBMC,the worse DCs function and maturity are, which would be one of important reasons of HBV persistent infection.