Study On The Therapeutic Mechanism Of Matrine On Aerobic Vaginitis

ObjectiveTo explore the antibacterial effect of Matrine on the common pathogenic bacteria of aerobic vaginitis including Staphylococcus aureus,Escherichia coli,Enterococcus faecalis and Streptococcus agalactiae,and compare with clindamycin.To detect the improvement of Matrine on the inflammation of vaginal epithelial cells stimulated by bacterial structural components,providing a preliminary study for the potential therapeutic effect of Matrine for aerobic vaginitis.MethodsUsing the microdilution method defined by the Clinical and Laboratory Standards Institute CLSI,according to previous literature on the pathogenic results of aerobic vaginitis,including Staphylococcus aureus,Escherichia coli,Enterococcus faecalis and Streptococcus agalactiae.Matrine and clindamycin were diluted in multiple concentrations to detect the minimum inhibitory concentration of these four bacteria.Diluting Matrine to different concentrations.Using CCK8 kit and spectrophotometer to detect the effect of different concentrations of Matrine on vaginal epithelial cell proliferation.And then stimulating VK2E6/E7 vaginal epithelial cells in appropriate concentration Matrine with or without lipopolysaccharide,peptidoglycan.Using Western Blot method to detect the activation of NF-κB signaling pathway and its alleviation.Results1.The minimum inhibitory concentration of Matrine on Staphylococcus aureus,Escherichia coli,Enterococcus faecalis and Streptococcus agalactiae are 15.5mg/mL,15.5mg/mL,7.7mg/mL,7.7mg/mL,respectively.2.Matrine could alleviate the up-regulation of NF-κB after LPS and PGN stimulation.ConclusionMatrine has a bacteriostatic effect on the common pathogens of aerobic vaginitis Matrine could alleviate the up-regulation of NF-κB after LPS and PGN stimulation.