Studies On Chemical Constituents Of The Viticis Fructus(Vitex Trifolia L.v A R.simplicifolia Cham.)

Vitex trifolia l.v.r.simplicifolia Cham is the dry and ripe fruit of Vitextrifolia l.In traditional Chinese medicine,the drug is often used in the solution of the surface,diverging wind heat,treating wind heat cold,headache head wind.Modern medical research has found that monophyletic vitex has significant pharmacological activities in the aspects of cytotoxicity,anti-oxidation,bacte-riostasis and anti-inflammation.In recent years,some studies have shown that the monophyletic nepenia l.is rich in chemical constituents,mainly including diterpenoids,flavonoids,lignans,iridoids,phenolic acids and other chemical constituents.In addition to some flavonoids with clear pharmacological activity,some diterpenes also show remarkable anti-tumor activity.In order to further develop and utilize simple vitex trifolia plant resources,better understanding of the composition of vitex trifolia plant chemical composition,pharmacological activity of more clearly grasp the unifoliate vitex trifolia material base,the integrated use of diatomite relief columns,including different particle size of silica gel column and gel column,octadecyl bonded silica gel column,such as all kinds of chromatographic separation technology,collocation,silica gel thinlayer chromatography and nuclear magnetic resonance spectrum detection means such as high resolution mass spectrometry separation and identification of the 30 compounds,Including lignans compounds(7,8 r)-dihydrodehydrodiconiferyl a-lcohol(1),3-(beta-D-glucopyranosyloxymethyl)-2-(4-hydroxy-3-methoxyphenyl)-5-(3-hydroxypropyl)-7-methoxydihydrobenzofuran(2),(7 r,r)-7,8-dihydro-9 ’-hydroxyl-3’-methoxyl-8-hydroxymethyl-7-(4-hydroxy-3-methoxyphenyl)-9 ’1’-benzofuranpropanol-O-beta-D-glucopyranoside(3),(7,8 r)-dihydrodehydrodiconiferyl Alcohol-4-O-beta-D-glucopyranoside7 R,8 s-erythro-dihydroxydehyd-rodiconi-feryl alcohol(4),(7 r,r)-7,8-dihydro-9 ’-hydroxyl-3’-methoxyl-8-hydroxymethyl-7-(4-hydroxy-3-methoxyphenyl)-1 ’-benzofuranpr-opanol 9 ’-o-lo-d-glucopyranoside(5),lawsonicin(6),(7S,8 r)-dih-ydrodehydrodiconiferyl alcohol(7),(8),erythreo Prunustosanan AI-8(7 r,r)-guaiacyl-glycerol-beta-O-4 ’-dihydroconiferyl Mr(9),threo-8(7 r,r)-guai-acyl-glycerol-beta-O-4’-dihydroconiferyl Ether(10),threo-(7R,8R)-guaiacyl-glycerol-o-4 ’-dihydroconiferyl ether(11),threo-(7R,8R)-guaia-cyl-glycero-l-o-4 ’-dihydroconiferyl ether(12),illiciumlignum an E(13),illiciumlignuman B(14),leptolepisol C(15),illiciumlignan F(16),Herpetin(17),a total of 17;Pedunculariside(18),Agnuside(19),pedunculariside(20).Viteagnuside A(21)vitexifolin(22)(rel 5S,6R,8R,9R,10S)-6-acetoxy-9-hydroxy-13(14)-labden-16,15-olidagnusid(23);Flavonoids Luteolin(24),1-hydroxyl-3,5,8-trimeth-oxyxanthone(25)1,7-dihydroxyl-3,8-dimethoxanthone(26),1,4,8-trimethoxant-hone-6-o-oxy-d-glucoronyl-(1→6)-o-oxy-d-glucoside(27);1 coumarin(-)-(3’S,4’S)-3’-acetoxy-4’-angeloyloxy-3’,4’-dihydroseselin(28);P-hydroxybenzoic acid B(29);Alkaloid compound compound1a(30).Compounds 4～17,25～27 were obtained from this plant for the first time.