The Effect Of Oxymatrine Combined With Curcumin Local Infusion On Restenosis After Balloon Injury Abdominal Aorta In Rabbits

Objective To explore the effect of local perfusion of oxymatrine and curcumin on restenosis after abdominal aortic balloon injury.Methods 1.Preparation and experimental group of rabbit abdominal aortic balloon injury of restenosis model: New Zealand male big-ear white rabbits(common grade)were selected.After pre-feeding for 1W,all experimental rabbits were given abdominal aortic balloon injury surgery and fed with high-fat diet for four weeks.Rabbits were given abdominal aorta angiography,and the rabbits who met the enrollment conditions of vascular stenosis were randomly divided into five groups.Which were blank control group(Control group,Not giving rabbit abdominal aorta balloon injury and feeding high-fat feed,General feed feeding),model group(Model group,Rabbits were given balloon injury of abdominal aorta and high-fat diet,0.9% Na Cl / 4m L),oxymatrin group(OMT group: Rabbit were given balloon injury of abdominal aorta and high-fat diet for 4W,OMT150 mg / 4m L),curcumin group(Cur group: Rabbit were given balloon injury of abdominal aorta and high-fat diet for 4W,Cur 0.1mg / 4m L),oxymatrin combined with curcumin group(OMT + Cur group: Rabbit were given balloon injury of abdominal aorta and high-fat diet for 4W,(OMT150mg + Cur 0.1mg)/ 4m L),Each group was divided into eight rabbits.After successful replication of the model,all the experimental rabbits were given local perfusion medicine treatment,and then fed with general feed for four weeks.All the experiments were performed with angiography,OCT and HE staining of rabbit abdominal aortic stenosis.2.Abdominal aorta angiography: measurement of the change in area stenosis rate(MLA%)after angiography.3.OCT of abdominal aortic stenosis: After OCT was performed,images of stenosis blood vessels were collected and the changes in intima thickness and area were measured and analyzed.4.HE staining of the blood vessels in the stenosis of the abdominal aorta:Observe the pathological changes of the stenosis of the abdominal aorta in rabbits under ordinary light microscope.5.Determination of the concentration of VEGF and IL-1β: After the drugs were administered to each group by local infusion balloon administration,New Zealand white rabbits were fed with Ordinary feed for4 W,and the expression levels of VEGF and IL-1β in the experimental rabbits were tested by ELISA.Results1.Animal model: After model rabbits were given balloon injury to the abdominal aorta and fed with high-fat diet for 4W,abdominal aorta angiography showed that the vascular area stenosis rate was between 20%and 80%.Pathological HE staining confirmed: blank rabbit: vascular endothelium,inner elastic plate is intact and unbroken,no thickening of the intima and media layers,no crystal deposition of lipids and cholesterol.Rabbits in the model group: HE staining of blood vessels in the stenosis of the abdominal aorta showed severe luminal stenosis and basic occlusion.Vascular stenosis of the blood vessels showed eccentric lesions;vascular endothelial rupture and incompleteness,thickening of the intima and intima layer,a large amount of visible Lipid and cholesterol crystal deposits.2.The change of MLA% in abdominal aortic angiography: The results showed that after 4W of abdominal aortic balloon injury plus high-fat feeding,At W4,the MLA% between the groups was at the same baseline level,and there was no statistical difference(P>0.05).At W8,the MLA% of the abdominal aortic stenosis in the OMT group,Cur group,and OMT + Cur group was significantly lower than that in the Model group(P <0.05).3.OCT observed the changes of vascular intima thickness and area of??stenosis in each group: The results showed that there was no statistically significant difference in the intimal thickness and intimal area of the stenotic segment of rabbit abdominal aorta between Control group,Model group and medication groups before W0(P>0.05).The intimal thickness and intimal area of the narrow segment blood vessels between the groups were at the same baseline level;at W4: the intimal thickness and area of the Model group increased significantly compared with the Control group(P <0.05),indicating that the replication balloon injury The narrow model succeeds.The intimal thickness and intimal area between the Model group and the OMT group,Cur group,and OMT + Cur group were not statistically significant(P>0.05),indicating that the Model group and the medication groups were on the same baseline;at W8,the intimal thickness and intimal area of the stenotic segment of abdominal aorta in the three-medicine group were significantly lower than those in the model group,and the OMT + Cur group decreased more significantly than the OMT and Cur groups(P<0.05).4.HE staining of stenotic blood vessels: The lumen of the control group was normal,and the endothelium of the blood vessel was smooth and intact,and there was no proliferation of the intima and media.Cholesterol deposition;the vascular lumen of the oxymatrine and curcumin groups had stenosis.At 100 x magnification,the endothelium and internal elastic plate of the blood vessel were partially broken,and the vascular smooth muscle cells and vascular smooth muscle cells of the stenosis Foam cells.The vascular lumen of the matrine combination medicine group was obviously enlarged,the inner elastic plate was basically unbroken,mostly continuous,and the media layer contained some smooth muscle cells and foam cells.The lumen of OMT + Cur group was larger than that of OMT group and Cur group.5.The levels of VEGF and IL-1β:(1)The concentration of VEGF in the Model group was significantly higher than that in the Control group(P<0.05).The serum VEGF in the OMT group,Cur group and OMT + Cur group was significantly higher than that in the Model group(P<0.05),and the OMT + Cur group Serum VEGF was higher than that in OMT group and Cur group(P<0.05).(2)IL-1β in Model group was significantly higher than that in Control group(P<0.05),IL-1β expression level in Model group was significantly higher than that in OMT group,Cur group and OMT + Cur group(P<0.05),The concentration of IL-1β in OMT + Cur group was significantly lower than that in OMT group and Cur group.Conclusion Rabbit abdominal aorta balloon injury vascular endothelium and high-fat diet feeding 4W can successfully replicate the model of rabbit abdominal aorta balloon injury restenosis model;local perfusion of matrine or curcumin can inhibit excessive proliferation of vascular intima and inhibit revascularization Narrow and no difference between the two,the combined effect of local infusion of two drugs to inhibit hyperplasia is better than one of them.