| Purpose Peripheral nerve injury(PNI)is one of the common clinical diseases.Micro RNAs(miRNAs)are closely related to the repair mechanism after peripheral nerve injury.We have found out the target miRNA and predicted its target genes through bioinformatics analysis in the preliminary study.In order to validate SLC20A2 and HSPA12 A are target genes of miR-136-5p,and study the function of miR-136-5p and its target genes in vitro,here we proceed the cytological experiments.Methods The miR-136-5p high expression plasmid and miR-136-5p low expression plasmid were constructed and transfected into the cultured neuronal cells to form the miR-136-5p high expression groups and miR-136-5p low expression groups,and the normal neuron groups were used as the control groups.After the above three groups of cells were subjected to RNA extraction and reverse transcription,PCR detection was performed to verify the predicted target genes SLC20A2 and HSPA12 A,and we study on the function of miR-136-5p and target genes in vitro by the Av / Pi test,Transwell test,and neuronal serotonin(5-HT).Results The results of PCR detection of target genes in neuronal cells showed that the expression levels of SLC20A2 and HSPA12 A were not significantly correlated with the expression levels of miR-136-5p.Av / Pi test results showed that the apoptosis level of miR-136-5p high expression groups was significantly increased.The detection of 5-HT level showed that the level of 5-HT in miR-136-5p high expression groups was significantly reduced,while that of miR-136-5p low expression groups was significantly increased.Transwell test results showed that two experimental groups had no significant difference in cell migration ability from the normal neuron groups.Conclusions SLC20A2 and HSPA12 A are not yet considered target genes for miR-136-5p.The increase of miR-136-5p will promotes neuronal apoptosis and inhibits the level of 5-HT,while has no significant effect on the cell migration ability. |
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