Role Of Estrogen-related Receptor α In Blue Light-induced Apoptosis Of Human Retinal Pigment Epithelial Cells

Objective 1.To observe the expression of ERRα in normal cultured ARPE-19 cells and the effect of blue light on it.2.To explore the role and mechanism of ERRα in apoptosis of human retinal pigment epithelial cells induced by blue light.Methods Establish blue light model: ARPE-19 cells were divided into two groups: control group(avoiding light)and blue light group with different light duration(2,4,6,8 h).The morphological changes of ARPE19 cells in each group were observed by inverted microscope,and the proliferation rate of ARPE19 cells in each group was detected by CCK-8 method.Western blot was used to detect the effect of blue light irradiation on the expression of cleaved-caspase3 and ERRα protein in ARPE-19 cells.2.The experiment was divided into control group(light avoidance group),XCT-790 group,blue light group and blue light +XCT-790 group,the apoptosis rate of ARPE-19 cells was detected by Tunel method,the expression of ERRα protein was detected by Western blot,and the levels of Bcl-2 and Bax mRNA in each group were detected by q PCR.Results1.The effect of blue light on the morphology of ARPE-19 cells: blue light can lead to abnormal cell morphology: round shape,shrinking volume,shrinking nucleus,transparent circle on the edge of the cell,suspended cells and cell fragments in the culture medium.2.The effect of blue light on the proliferation rate of ARPE-19 cells: compared with the control group,the cell proliferation rate of all blue light groups decreased,and further decreased with the increase of light duration.3.The effect of blue light on the expression of cleaved-caspase3 and ERRα protein: compared with the control group,the relative expression of cleaved-caspase3 protein increased significantly from 2 hours(P < 0 01),and increased with the increase of light duration(P < 0 01).Compared with the control group,the expression of ERRα in all light groups increased,and the expression continued to increase with the increase of light duration,and the protein content was the highest after 6 hours of light exposure.4.The role of ERRα blue light in inducing apoptosis of human retinal pigment epithelial cells:compared with the control group,the apoptosis rate of XCT-790 was not significantly different from that of the control group,but the apoptosis rate of blue light group was higher than that of blue light group,and the apoptosis rate of blue light + XCT-790 group was higher than that of blue light group(P < 0.01).5.A preliminary study on the mechanism of ERRα inhibiting apoptosis of human retinal pigment epithelial cells induced by blue light: compared with the control group,the expression of ERRαprotein in XCT-790 group was decreased,and the expression of ERRα in blue light + XCT-790 group was significantly lower than that in blue light group(P < 0.01).At the same time,under normal culture conditions,XCT-790 had no effect on the expression of Bcl-2 and Bax mRNA.Under blue light induction,XCT-790 had no effect on the expression of Bax mRNA,but further decreased the expression of Bcl-2mRNA.Conclusion1.Low expression of ERRα protein in normal cultured ARPE-19 cells2.Blue light induced increased apoptosis of ARPE-19 cells and increased expression of ERRαprotein at the same time.3.ERRα could reduce the apoptosis of ARPE-19 cells induced by blue light.4.Under blue light induced conditions.,ERRα may play an anti-apoptotic effect by promoting the expression of Bcl-2 in ARPE-19 cells.