| Objective: The purpose of this experiment is to observe the effects of Tongli Sanjiao Qingre Lishi prescription on the expression of monocyte chemoattractant protein-1(MCP-1),macrophage migration inhibitory factor(MIF)and intercellular adhesion molecule-1(ICAM-1)in renal tissue of Ig A nephropathy rats,and explore the possible therapeutic mechanism of Tongli Sanjiao Qingre Lishi prescription in treating Ig AN rats.It provides experimental basis for the study and safe application of Ig A nephropathy.Methods: 48 male healthy and clean SD rats with a body weight of150-180 g were randomly split up into 4 groups: normal group(N group),model group(M group),benazepril group(B group)and traditional Chinese medicine group(Z group).After 1 week of adaptive rearing in the scientific research laboratory,the Ig AN rat model was established by the immune complex method of bovine serum albumin,lipopolysaccharide and Subcutaneous injection of carbon tetrachloride and castor oil.After the successful preparation of the model,the four groups were treated for 4 weeks.At different stages of the experiment,rats were placed in a metabolic cage to detect 24-hour urinary protein(24h-UTP).The rats were sacrificed at the 14 th week,and serum and renal tissue samples were taken to detect the content of biochemical indicators in each group,and the expression changes of MCP-1,MIF and ICAM-1 in renal tissue.Result:1.In this experiment,group N rats were in good condition,but group M,group B and group Z showed disordered skin and hair gradually lost luster,mental deterioration,no gain or even loss of weight after modeling.after the corresponding drug treatment,the spirit,diet,weight and hair color of group B and group Z were better than those in group M.There was no death in the process of the experiment.At the end of the experiment,there were 12 rats in group N,12 in group M,12 in group B and 12 in group Z.2.At the end of 10 weeks,compared with group N,24-hour urinary protein(24h-UTP)in group M,B and Z increased significantly(P<0.01);at the end of 14 weeks;compared with group N,24h-UTP in group M was significantly higher(P<0.01);compared with group M,the 24h-UTP of the rats in group B and group Z decreased significantly(P<0.01).There was no significant difference in 24h-UTP of the rats between group B and group Z(P >0.05).3.There was no significant difference in serum creatinine(Scr),urea nitrogen(BUN),plasma albumin(ALB),triglyceride(TG)between N,M,B and Z groups(P>0.05).The total cholesterol(TC)in group M was significantly higher than that in group N(P<0.01);Compared with group M,the expression of group B and group Z decreased(P<0.05),and there was no significant difference between group B and group Z(P>0.05).4.The renal structure of group N was clear and no abnormal pathological changes were observed by light microscopy,electron microscopy and immunofluorescence.The pathological damage of group M was the most significant.Compared with group M,the pathological injury in group B and group Z was significantly improved to some extent.5.The expression of ICAM-1 by immunohistochemistry and MCP-1 and MIF by Western blot was observed in the kidney of rats.Compared with group N,the expression of group M was significantly increased(P<0.01);compared with group M,the expression of group B and group Z decreased(P<0.05);there was no significant difference between group B and group Z(P>0.05).6.The expression of MCP-1 m RNA,MIF m RNA and ICAM-1 m RNA were observed by RT-PCR in renal tissue of rats.Compared with group N,the expression in group M was significantly increased(P<0.01);Compared with group M,the expression in group B and group Z was decreased(P<0.05);there was no significant difference between group B and group Z(P>0.05)7.The expression of MCP-1,MIF and ICAM-1 in the blood of the rats were observed by ELISA.Compared with group N,the expression of MCP-1,MIF and ICAM-1 in the serum of group M was significantly increased(P<0.01).Compared with group M,the expression of MCP-1,MIF and ICAM-1 in group B and Z was decreased(P<0.05).There was no significant difference between group B and group Z(P>0.05).Conclusion:1.Tongli Sanjiao Qingre Lishi priscription can significantly reduce the24-hour urinary protein content of Ig A nephropathy rats,and protect the structure and function of kidney,its effect is equivalent to benazepril.2.Tongli Sanjiao Qingre Lishi priscription can obviously reduce the pathological changes of Ig A nephropathy,and alleviate the progress of Ig A nephropathy,its curative effect is equivalent to benazepril.3.Tongli Sanjiao Qingre Lishi priscription can significantly reduce the expression of MCP-1,MIF and ICAM-1 in the renal tissue of Ig A nephropathy rats,decrease its expression in serum,reduce the local infiltration of inflammatory cells in the kidney,and reduce the proliferation and reconstruction of mesangial cells and extracellular matrix of Ig A nephropathy,restore the immune balance,delay the process of the disease,and its effect is equivalent to benazepril. |
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