Screening Of Active Components Of Sparganium And Its Effect On Proliferation And Apoptosis Of Gastric Cancer MGC-803 Cells

PurposeObjected to reveal the mechanism of the effects of Sparganium in gastric cancer.MethodsWe employed network pharmacology to analyze the active ingredients and potential pathways of Sparganium in gastric cancer.In order to evaluated the reliability of the key targets and pathways,we used MTT assay,flow cytometry,real-time quantitative polymerase chain reaction and western blot.Results1.Employed by network pharmacology,five active ingredients for gastric cancer treatment were selected,including formononetin,stigmasterol,beta-sitosterol,hederagenin,transgondoic acid.2.Twenty-eight overlapping gene symbols related to gastric cancer and drugs were obtained from Gene Cards and OMIM databases,main including CASP3,ERS1,AR,CASP8,CASP9.3.We suggested that apoptosis signaling pathway and P53 signaling pathway in gastric cancer might serve as the key points and principal pathways for gastric cancer treatment.4.Five active ingredients of Sparganium were detected cell proliferation in gastric cancer using the MTT assay.The results showed that the components could inhibit the proliferation of gastric cancer MGC-803 cells and the effects were in a dose dependent manner.In addition,formononetin,stigmasterol,beta-sitosterol,hederagenin,transgondoic acid of Sparganium inhibited the proliferation of gastric cancer MGC-803 cells with half maximal inhibitory concentration value at 24 h were respectively 52.94μM/L,50.64μM/L,29.67μM/L,55.82μM/L,5089.40μM/L.Meanwhile,5-FU inhibited the proliferation of MGC-803 cells with half maximal inhibitory concentration value at 24 h was10.70μM/L.5.In comparison with the control group,five active ingredients in Sparganium could induce the apoptosis in gastric cancer MGC-803 cells by using flow cytometry(P<0.05).6.Compared with the control group,five active ingredients in Sparganium could elevate Caspase3 and Caspase9 gene expression in gastric cancer MGC-803 cells.Five active ingredients except for transgondoic acid in Sparganium could also elevate Fas and Bax gene expression.In addition,formononetin,beta-sitosterol,stigmasterol and Sparganium compound were elevated Bcl-2 gene expression in gastric cancer MGC-803 cells,while transgondoic acid and hederagenin were decreased(P<0.05).7.Compared with the control group,five active ingredients in Sparganium could elevate Caspase3 and Caspase9 protein expression in gastric cancer MGC-803 cells.Five active ingredients except for transgondoic acid in Sparganium could also elevate Fas and Bax protein expression.In addition,formononetin,beta-sitosterol,stigmasterol and Sparganium compound were elevated Bcl-2 protein expression in gastric cancer MGC-803 cells,while transgondoic acid and hederagenin were decreased(P<0.05).ConclusionFive active ingredients including formononetin,stigmasterol,beta-sitosterol,hederagenin,transgondoic acid in Sparganium all could inhibit the proliferation of gastric cancer MGC-803 cells.The protential mechanism might induce the apoptosis of human gastric cancer MGC-803 cells through apoptotic signaling pathways.