| Sponge associated microorganisms are important sources for novel natural bioactive compounds. In this study, two sponge-associated fungi, 9-1 and 10-7 were isolated respectively from South China Sea sponges Plakortis simplex and Hippospongia canaliculata var.cylindrica. After observation of their morphological characteristics and analysis of 600-bp 18S rDNA, 9-1 was identified as Penicillium sp., 10-7 was identified as Asperqillus sp.. These two fungi both showed evident activity against Staphylococcus aureu. Also in our previous research, a Streptomyces sp.DA18 was isolated from marine sponge Craniella austrialiensis, which was found to have multiple antimicrobial activities.Through thin-layer chromatography, gel exclusion chromatography, silica column chromatography, reverse HPLC etc., sixteen compounds were isolated from the EtOAc extracts of those three mentioned strains. The structures of all the compounds were elucidated by 1D-NMR and 2D-NMR and MS spectra.Compound 1-1 to 1-3 from Penicillium sp. 9-1, 1-1: Ergosterol peroxide, 1-2: a unsaturated fatty acid with at least 10 carbon atoms, 1-3: Methyl ester derivative of 1-2.Compound 2-1 from Asperqillus sp. 10-7: 5-hydroxymethyl-furoic acid.Compounds 3-1 to 3-13 from Streptomyces sp. DA18, 3-1: Cyclo-(L-Pro-D-Phe), 3-2: Cyclo-(D-Pro-D-Phe), 3-3: Cyclo-(D-6-Hyp-L-Phe), 3-4: Cyclo-(L-Pro-D-Val), 3-5: Indole-3-carboxaldehyde, 3-6: Indole-3-carboxylic acid, 3-7: Uracil, 3-8: Thymine, 3-9: Guanine, 3-10:β-Carboline, 3-11: Cholest-3, 6, 7-triol, 3-12: Isolumichrome, 3-13: P- hydroxybenzoic acid.Some literatures have documented that compound 2-1 exhibited bioactivity against Staphylococcus aureu and compound 3-2 conducted as antibiotic against Vibrio anguillarum, which suggests that sponge-associated microorganisms play a considerable role in the chemical defence of their host sponge. Analysis of pharmacological activities of some of the isolated compounds is being carried out. |
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