MiR-1-3p-ETS1 Regulates Cyclic Strech-induced Proliferation Of Vascular Smooth Muscle Cells

Mechanical stretch modulates proliferation of vascular smooth muscle cells(VSMCs),which plays an important role in the pathogenesis of hypertension.But the underlying mechanisms are unclear.We investigated the role of microRNA-1-3p(miRNA-1-3p)in proliferation of VSMCs induced by mechanical cyclic stretch.Our data show that miRNA-1-3p is downregulated in the aorta of the spontaneous hypertension rat(SHR).The pathological mechanical stretch at 15%suppresses the expression of miRNA-1-3p,calponin and SM22,but enhances proliferation of VSMCs as well as expression of V-ets erythroblastosis virus E26 oncogene homolog 1(ETS-1),collagen type Ⅰ alpha(Col-1a),collagen type Ⅲ alpha(Col-3a)and elastin.The overexpression of miRNA-1-3p inhibits cell proliferation and induces expression of calponin and SM22,but decreases the expression of ETS-1,Col-1a,Col-3a and elastin.Whilst the overexpression of ETS-1 enhance cell proliferation.Mechanical stretch at 15%combination with losartan treatment increase the expression of miRNA-1-3p,calponin and SM22,but decrease the expression of ETS-1,Col-la and Col-3a.Dual luciferase reporter assays revealed ETS-1 as a direct target of miRNA-1-3p.These findings suggest that miRNA-1-3p regulates VSMC function through the regulation of ETS-1 during hypertension induced vascular remodeling.MiRNA-1-3p may be viable therapeutic targets for hypertension.Figure(seven)table(one)reference(forty-six)...