Experimental Study On Transformation Of The Animal Model Of Cold Bi Syndrome To Heat Bi Syndrome In Rheumatoid Arthritis

BackgroundRheumatoid arthritis(RA)is an autoimmune disease with synovitis as the center.It belongs to the category of Chinese medicine arthralgia and is also the representative disease of arthralgia.There are two types of RA active stage.Is a joint swelling and pain,but not red,though touch is not hot,swelling degree can be light to heavy,can you touch the synovial thickening,this belongs to the Chinese medicine was winter a large number of experiments have shown that Collagen induced arthritis model(Collagen-induced arthritis and the CIA)model belongs to the TCM type of heat treatment of Human Tumor Necrosis Factor alpha(the Human Tumor Necrosis Factor alpha,hTNF alpha)transgenic mice(Tgtc)is in the background of FVB mice hTNF alpha gene expression,can appear spontaneouslyProgressive joint swelling deformation,and its pathological prompt joint with a large number of synovial hyperplasia and progress of cartilage erosion Characteristics of bone destruction,the performance is similar to的 human was winter,and our previous study found that environmental and aconite decoction can relieve rheumatic fever Tgtc arthritis symptoms in mice Clinical RA patients with arthritis often cannot,by a single card type represents fever transformationFever,but the current animal models of RA can only simulate the single card type,we hope that on the basis of hTNF alpha transgenic mice was winter building acute arthritis induced by the CIA,which was not the conversion between But because Tcra FVB mice-V11.1 coding sequence polymorphism and Tcrb-this gene is missing,leading to resistance to the CIA So the experiments with the DBA/1 mice hybridization is sensitive to the CIA,and then to the CIA for its F1 induction module,CWhether the induction can induce acute inflammatory response on the basis of HTNFa transgenic mice arthritis,so as to establish an animal model of the transformation from cold to heat,so as to better simulate the complex syndromic changes of human RAContentChapter 1 Effect of type 2 collagen induction on arthritis in hTNFαtransgenic mice and its mechanismObjectiveTo investigate whether bovine type II collagen(CII)induction can induce acute arthritis manifestations on the basis of hTNFa transgenic mice arthritis,establish an animal model of cold Bi to heat Bi transformation,and explore its Methods:hTNF alpha transgenic mice(FVB)background and DBA/1 mice hybrids F1 generation of mice,the gene identification is divided into hTNF alpha gm(hTNF alpha+)and wild type(WT),the CIA model,were divided into model group(CIA)and normal group(NC),a total of four groups,the observation records of each joint diameter of arthritis index change of general performance in mice serum cattle CII specific IgG Elisa method,and the content of IgG2a,ankle joint line HE dyeing TRAP microessel-density counts and immunohistochemical detectionResults:After CIA modeling,hTNFα+mice could produce specific IgG and IgG2A antibodies,but did not show acute diffuse joint swelling similar to WT CIA group.The main manifestations were joint deformity,which presented progressive aggravation,accompanied by mild to moderate swelling,and no obvious redness.Pathological suggest broad synovial cell hyperplasia and bone erosion,a large number of angiogenesis,moderate inflammatory infiltration,and the subchondral bone and bone-synovial border exist a large number of macrophages and osteoclast CIA building after hTNF arthritis clinical manifestations of alpha+mice inflammatory infiltrates things no significant change(P>0.05),but the macrophages in the synovial membrane and osteoclast number increase bone damage increased slightly(P<0.05)Conclusion:Type ii collagen can’t aggravate hTNF alpha+mice after induction in arthritis clinical manifestation and inflammatory infiltration degree,but may be through specific CII macrophages in the production of antibodies and the promotion of synovial membrane and osteoclast number increase,increase the bone destructionChapter 2 Explorations of other classical models of induced arthritis in hTNF transgenic miceObjective:To explore whether CAIA and PGIA modeling methods can induce acute arthritis manifestations on the basis of HTNFa transgenic mice arthritis,and establish the animal model of transformation from cold to heat.Methods:CAIA model,PGIA model and CIA model were established on the basis of HTNFa transgenic mice(TGTC).The corresponding control group was set up to observe the changes of joint diameter,arthritis index and other general manifestations of mice in each group,to observe whether acute arthritis symptoms could be induced.Results:After CAIA,PGIA and CIA model,TGTC mice showed acute arthritis.Conclutions:The classical RA model building methods can not induce acute arthritis of TGTC arthritis mice,and can not aggravate the joint inflammation of TGTC mice.