| Preeclampsia is a newly discovered hypertensive disease after 20 weeks of gestation with unknown pathogenesis and it is a series of clinical syndrome characterized by multiple organ dysfunction.The incidence of preeclampsia is as high as 2-8%during pregnancy.ELABELA(ELA),as a new type of APJ endogenous peptide ligand,which is mainly distributed in human placenta and kidney.ELA-APJ signal pathway is involved in the formation of cardiovascular system in early embryo.Low expression of ELA or knockout of ELA gene can cause abnormal placental angiogenesis,hypertension,proteinuria and other symptoms.However,there are few studies on the expression of ELA in peripheral circulation in the first trimester pregnancy and its correlation with preeclampsia.[Research Objectives]The purpose of this study is to explore the correlation between preeclampsia and serum ELA by detecting the maternal peripheral circulating ELA level in early pregnancy,so as to provide a new theoretical basis for the prediction and prevention of prceclampsia.[Research methods]Subjects:(1)Preeclampsia group:randomly selected 41 patients with preeclampsia who received maternal and child health manual from December 2019 to July 2020 in Our Hospital,owning complete pregnancy data and later hospital delivery informations;(2)non-preeclampsia group:Forty-seven patients with non-preeclampsia who were registered and delivered in hospital in the same period were randomly selected.Methods:fasting peripheral venous blood was collected in the first trimester(11-13 weeks).After centrifugation,the upper serum was collected and stored in the refrigerator at minus 80℃.After the samples were collected,the maternal serum ELA level was determined by enzyme-linked immunosorbent assay(ELISA).Statistical methods:SPSS 25.0 software was used to analyze the data,and if P value<0.05,it was considered to be statistically significant.[Research results]1.Comparison of general data:there was no significant difference in age,frequency of pregnancy and education between the two groups,but the pre-pregnancy weight,pre-pregnancy BMI and pre-delivery weight in the preeclampsia group were significantly higher than those in the non-preeclampsia group(P<0.05).2.Comparison of delivery outcomes:compared with non-preeclampsia group,pregnant women with preeclampsia had higher admission mean systolic blood pressure,diastolic blood pressure and cesarean section rate,earlier gestational age,smaller neonatal body length and weight,and lower Apgar score at 1 min after birth,with statistical significance(P<0.05).3.The median level of maternal ELA in early pregnancy was 1.06(0.62-2.12)ng/mL in the non-preeclampsia group and 1.77(1.25-3.46)ng/mL in the preeclampsia group,and the difference was statistically significant(P<0.05).Compared with the two groups,the ELA level of the preeclampsia group was higher than that of the non-preeclampsia group.4.There was a positive correlation between maternal circulating ELA level in early pregnancy and admission systolic blood pressure before delivery(rs=0.355,P<0.05).Bariate Logistic regression analysis showed that serum ELA level in early pregnancy combined with pre-pregnancy body mass index(BMI)could increase the predictive efficacy of preeclampsia.[Conclusion]1.A certain level of ELA was expressed in the circulation of non-preeclampsia and preeclampsia in early pregnancy.2.In early pregnancy,there was a difference in the expression of circulating ELA between preeclampsia and non-preeclampsia,and the expression of ELA in preeclampsia was h igher than that in non-preeclampsia.3.The increase of ELA level may be an evaluation index of preeclampsia,and ELA may be involved in the occurrence and development of preeclampsia. |
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