Expression Of OIP5 Gene In Ovarian Cancer And Its Clinical Significance

Background: Ovarian cancer is a common malignancy of female reproductive organs.Due to its complex histological types,lack of specific symptoms and effective early diagnosis methods,most patients are found in the late stage.Ovarian cancer has a high degree of malignancy and is easy to spread and metastasize in the pelvis and abdominal cavity.Most patients will relapse after initial treatment,and the 5-year survival rate is far lower than that of other tumor patients.OIP5 is an important tumor-associated antigen,which has been found to be restricted in normal tissues but widely expressed in most tumor tissues.It is closely related to the malignant biological behavior of tumor cells,such as proliferation and invasion,and is an independent risk factor affecting the prognosis of some tumor patients.Therefore,we speculate that OIP5 may be involved in the occurrence and development of ovarian cancer.Objective: This topic is aimed at studying OIP5 gene expression in ovarian cancer,and by regulating OIP5 in vitro ovarian cancer cell line(OVCAR-3)to further observe the effects of OIP5 on the proliferation,migration and other biological functions of ovarian cancer cells.To explore the role and clinical significance of OIP5 in ovarian cancer,in order to provide a new theoretical basis for the clinical diagnosis and treatment of ovarian cancer.Methods: In this study,28 cases of ovarian cancer patients treated in the Department of Obstetrics and Gynecology of Yijishan Hospital were selected as the research objects,and 35 cases of normal ovarian tissue were selected as the experimental control group.Using q RT-PCR technique to detect the m RNA expression level of OIP5 gene in ovarian cancer tissues and normal ovarian tissues.Using Western Blot technique to detect the protein expression level of OIP5 gene in ovarian cancer tissues and normal ovarian tissues.At the same time,immunohistochemical staining method was used to detect the OIP5 expression in ovarian cancer tissues and normal ovarian tissues.Finally,we statistically analyzed whether there is any difference.OVCAR-3 cell line was cultured in vitro,and si RNA transfection was used to down-regulate the expression of OIP5 gene in the cell line.We detected the down-regulation efficiency of OIP5 by q RT-PCR,the proliferation ability of ovarian cancer cells by CCK-8 method,and the invasion and migration ability of ovarian cancer cells by Transwell method.Results: 1.q RT-PCR,Western blot and immunohistochemistry to detect the expression of OIP5 gene in ovarian cancer tissues:(1)The m RNA expression level of OIP5 gene in ovarian cancer and normal ovarian tissue was detected by q RT-PCR,and the results were 2.90±0.90 and 1.39±0.88,respectively.The m RNA expression level of OIP5 gene in ovarian cancer group was higher than that in normal control group,and the difference was statistically significant(P<0.05).(2)Western blot to detect the protein expression level of OIP5 gene in ovarian cancer and normal ovarian tissues,the results were 3.27±0.73,1.71±0.52,respectively.Compared with the control group,the protein expression level of OIP5 gene in the ovarian cancer group was higher,the difference was statistically significant(P<0.05).(3)Immunohistochemical results showed that the expression of OIP5 protein was localized in the cell membrane and cytoplasm.Compared with normal ovarian tissue,the positive expression of OIP5 in ovarian cancer tissue was significantly up-regulated.2.The sequence with the highest down-regulation efficiency interfering with OIP5 gene expression was screened by q RT-PCR.Compared with the negative control group,si OIP5-3transfected showed an advantage in inhibiting OIP5 m RNA expression(P<0.05).3.Effects of OIP5 knockout on biological function of ovarian cancer OVCAR-3 cells:(1)In the cell proliferation test(CCK8 method),compared with the control group,the OD values measured in the OIP5-si RNA group were lower,indicating that the cell proliferation ability was inhibited,and the difference was statistically significant(P<0.05).(2)In the cell migration and invasion test(Transwell method),compared with the control group,the number of cells migrated and invaded in the OIP5-si RNA group decreased,suggesting that both the migration and invasion abilities of cells were inhibited,and the differences were statistically significant(P<0.05).Conclusion: The expression of OIP5 in ovarian cancer tissue was higher than that in normal ovarian tissue,suggesting that OIP5 may be involved in the occurrence and development of ovarian cancer.The down-regulation of OIP5 reduced the proliferation,migration and invasion ability of ovarian cancer cells,suggesting that OIP5 may promote the development and metastasis of ovarian cancer.The further study of OIP5 gene in ovarian cancer provides a new idea and target for gene therapy in clinical ovarian cancer.