Study On The Mechanism Of YAP/FOXM1 Pathway In Acute Asthmatic Induced By Viral Infection

Objective: To explore the effect of YAP/FOXM1 pathway in acute asthmatic induced by viral infection.Methods: Sixteen healthy and SPF Balb/c female mice aged 6-8 weeks were divided into control group,asthma group,YAP induced group and YAP inhibited group according to randomly.The model of asthma was established with house dust mite,acute asthma attack was induced by Poly(I:C),and the expression of YAP was selectively inhibited by verteporfin.The lung tissue of mice was retained.The m RNA and protein expressions of YAP and FOXM1/SPDEF/Muc5 AC were determined by real-time PCR and immunohistochemistry.Results: 1.The m RNA and protein expressions of YAP in asthma group were significantly higher than those in control group,and the difference was statistically significant(P<0.05);The YAP induced group was significantly higher than the normal control group,asthma group and YAP inhibited group(all P<0.05).2.The m RNA and protein expressions of mucus secretion-related transcription factor FOXM1 and downstream target genes SPDEF and Muc5 AC in asthma group were significantly higher than those in control group(P<0.05);The YAP induced group was significantly higher than normal control group,asthma group and YAP inhibited group(all P<0.05).3.The m RNA expression of IL-4 and IL-13 in asthma group was significantly higher than that in control group(P<0.05);The YAP induced group was significantly higher than control group,asthma group and YAP inhibited group(all P<0.05).The m RNA expression of IFN-γ in asthma group was significantly lower than that in control group(P<0.05).The YAP inducted group was significantly higher than that in control group,asthma group and YAP inhibited group(all P<0.05).Conclusion: FOXM1 is regulated by YAP in bronchial airway epithelium.The expression of FOXM1/SPDEF/Muc5 AC are further upregulated by activating YAP When the virus infests asthmatic mice,which promotes the goblet formation of bronchial epithelial cells,hypersecretion of mucus,aggravation of airway inflammation and induces asthma attack.