| Human life in the environment full of free radicals,our health and free radicals closely related,under normal circumstances,the generation of free radicals and eliminate keep dynamic balance,and participate in different sports training will produce different amount of free radicals,unreasonable physical exercise will lead to the occurrence of sports injury,such as exercise fatigue,aging of red blood cells.In sports training,how to quickly eliminate the discomfort caused by sports and how to improve sports performance is of practical significance to the improvement of athletic ability and the reduction of injury.Tin caulis dendrobii is one of the important varieties of traditional Chinese medicinal dendrobium stem,have great medicinal value,this experiment with metal caulis dendrobii polysaccharide mice intervention to participate in sports training,study the tin caulis dendrobii polysaccharide’s influence on the movement of mice liver antioxidant gene expression,for tin caulis dendrobii polysaccharide which provides the theoretical foundation,as a kind of natural antioxidant.Objective:In this experiment,Dendrobium officinale polysaccharide was used as an intervention condition.Some experimental mice were given Dendrobium officinale polysaccharide by gavage.The experiment lasted for six weeks.To reflect the antioxidant capacity of Dendrobium officinale polysaccharide,so as to find a beneficial way for the health of human body and improve the antioxidant capacity of athletes.METHODS: The experimental subjects were 96 8-week-old male Kunming mice,with the body weight of 36.21±4.21 grams,purchased from the Animal Experimental Center of Shandong University.They were randomly divided into normal saline control group(n=24),normal saline +exercise group(n=24),polysaccharide group(n=24)and polysaccharide + exercise group(n=24),and then divided into two weeks group,four weeks group and six weeks group.Gavage regimen:Adjust the dose according to the body weight of mice every week,and give 200mg/kg Dendrobium officinale polysaccharide or normal saline gavage 2 hours before training.The mice were formally trained 6 days a week,with a certain amount of exercise per day,one day of rest,and adaptive feeding for 7 weeks.Starting from 10 minutes in the first week,increasing to 30 minutes at the weekend,increasing by 30 minutes each week from the second week,and exercising for 3 hours in the sixth week.Six mice were randomly selected to collect their serum and liver after the end of training at the second,fourth and sixth weekend,respectively.The contents of AST and ALT in serum were detected by ELISA,and the expression levels of SODmRNA and GSH-PxmRNA in liver were detected by RT-PCR.Result:1.ALT: There was no significant difference between the two groups after two weeks.ALT concentration of mice in the four-week normal saline group was higher than that of mice in the polysaccharide exercise group,and there was a significant difference(P<0.05).ALT concentration of mice in the saline and saline exercise group was higher than that of mice in the polysaccharide group at 6 weeks,and the difference was significant(P<0.05).There was no significant difference in ALT concentration between weeks in the polysaccharide group,saline group and polysaccharide exercise group.ALT concentrations were significantly lower in the four weeks of saline exercise than in the six weeks of saline exercise(P<0.05).2.AST: The AST concentration in normal saline group for two weeks was higher than that in polysaccharide group and polysaccharide exercise group,and the difference was significant(P<0.05).AST concentration in normal saline group was higher than that in polysaccharide group,polysaccharide exercise group and normal saline exercise group,and there were significant differences(P<0.05).AST concentration in polysaccharide group for 6 weeks was significantly lower than that in normal saline group and polysaccharide exercise group(P<0.05),and extremely significantly lower than that in normal saline exercise group(P<0.01).There was no significant difference between the polysaccharide group and the normal saline group during the whole experiment cycle.AST concentration in polysaccharide exercise group for 2 weeks was significantly lower than that in group for 6 weeks(P<0.05).AST concentration in normal saline group for 2 weeks was significantly lower than that in 6 weeks group(P<0.05).3.SODmRNA: The level of SOD expression in the saline group was lower than that in the polysaccharide exercise group,and the difference was significant(P<0.05).The level of SOD expression of mice in the four groups was significantly lower than that of mice in the polysaccharide group,normal saline exercise group(P<0.01).The expression of SOD in four weeks polysaccharide group was significantly lower than that in polysaccharide exercise group(P<0.05).The SOD expression level of mice in saline group was significantly lower than that of mice in polysaccharide group and polysaccharide exercise group(P<0.05).The level of SOD expression in the polysaccharide group at 2 weeks was significantly lower than that in the polysaccharide group at 4 weeks(P<0.01),significantly lower than that of mice in the polysaccharide group at 6 weeks(P<0.05).The normal saline group was stable in the whole experiment period,and there was no significant difference between the weeks.The SOD expression level of mice in the polysaccharide exercise group for two weeks was significantly lower than that of mice in the four-week group(P<0.01),significantly lower than that of 6-week mice(P<0.05).The SOD expression level of mice in the saline group was significantly lower than that of mice in the 4-week group and 6-week group(P<0.01).4.GSH-PXmRNA: The GSH-PX expression level of mice in the saline group was lower than that of mice in the polysaccharide exercise group at 2 weeks,and the difference was significant(P<0.05).The GSH-PX expression level of mice in the four weeks normal saline group was significantly lower than that of mice in the polysaccharide group and normal saline exercise group(P<0.05).The GSH-PX expression level of mice in saline group was lower than that of mice in polysaccharide group and polysaccharide exercise group at 6 weeks,and there was significant difference(P<0.05).The GSH-PX expression level of rats in polysaccharide group,polysaccharide exercise group and normal saline exercise group fluctuated,but there was no significant difference between the two groups.The normal saline group was stable in the whole experiment period without obvious fluctuation.Conclusion: Dendrobium officinale polysaccharide inhibited the increase of serum AST and ALT concentrations in mice,indicating that it had a protective effect on liver.Dendrobium officinale polysaccharide can promote the expression of SODmRNA and GSH-PXmRNA in the liver of mice and improve the antioxidant capacity of the organism.Moderate exercise also has positive effects on the body,promoting the expression of SODmRNA and GSH-PXmRNA,inhibiting the release of AST and ALT,and excessive exercise reduces the expression of antioxidant enzymes,with negative effects. |
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