Expression Of LGALS1 And FN1 In Epithelial Ovarian Cancer And Its Clinical Significance

Background and Purpose:Epithelial ovarian cancer(Epithelial ovarian cancer,EOC)is one of the most fatal gynecological cancers.It ranks seventh among female malignant tumors in the world,with 230,000 nealy diagnosed cases each year,of which 150,000 die of the disease.The 5-year survival rate after diagnosis is 46%.Therefore,we should deeply study the mechanism of EOC,understand the occurrence and development of the disease,and strive to find the potential therapeutic targets of EOC,so as to provide instructions for the prevention of recurrence and drug resistance,so as to improve the survival rate of patients.It is known that soluble galactoside-binding lectin 1(Lectin,galactoside-binding,soluble1,LGALS1)exists in and out of cells and participates in a variety of pathophysiological processes in vivo.It has been found that LGALS1 protein is highly expressed in a variety of malignant tumors and mediates the migration and invasion of tumor cells by regulating the epithelial-mesenchymal transformation(Epithelialmesenchymaltransition,EMT)of malignant tumors.Fibronectin 1(Fibronectin1,FN1)has a wide range of biological activities and plays a certain role in regulating cell growth,adhesion,migration and so on.It has been found that as a surface marker of interstitial cells,it plays an important role in the process of EMT.In this study,we observed the expression of LGALS1 protein and FN1 protein in EOC,analyzed the correlation between LGALS1 protein and FN1 protein,explored the role of LGALS1 protein in EOC,and analyzed the relationship between LGALS1 and age of onset,pathological type,pathological grade,clinical stage,lymph node metastasis,serum CA125 and HE4 levels in patients with EOC.Methods:1.By Oncomine database and Kaplan-Meier database,the expression of LGALS1 m RNA in ovarian cancer is observed,and the correlation between the expression of LGALS1 m RNA and the overall survival time((Overall survival,OS))and progression-free survival((Progression free survival,PFS)of ovarian cancer is analyzed.2.From July 2020 to December 2020,43 tissue of EOC case and 29 cases of normal ovarian case confirmed by ovariectomy due to gynecological benign diseases are collected in the second Hospital of Jilin University from July 2020 to December 2020.The expression levels of LGALS1 protein and FN1 protein in pathological tissues of the patients are detected by immunohistochemical staining(Immunohistochemistry,IHC).The basic information table of clinic pathological features of EOC patients is drawn,and the relationship between the expression level of LGALS1 protein and the age of onset,pathological type,pathological grade,clinical stage,lymph node metastasis,serum CA125 and HE4 levels of EOC patients is analyzed.3.SPSS25.0 statistical software is used for data statistical analysis.The counting data are expressed as the number of cases(percentage),chi-square test is used to compare the two groups,Spearman method is used to analyze the correlation between LGALS1 and FN1 protein expression.Results:1.The analysis of Oncomine database showed that the level of LGALS1 m RNA in ovarian cancer tissues is significantly higher than that in normal ovarian tissues and borderline ovarian epithelial-stromal tumors(P < 0.001,P < 0.01).Through Kaplan-Meier database analysis,it was found that OS and PFS are significantly shortened in EOC patients with high expression of LGALS1(P < 0.001,P < 0.001).2.The results of IHC show that the expression rate of LGALS1 protein and FN1 protein in EOC tissue is higher than that in normal ovarian tissue,and the expression of LGALS1 protein and FN1 protein in EOC tissue is significantly higher than that in normal ovarian tissue(P <0.05,P < 0.01).3.The correlation between the expression of LGALS1 protein and FN1 protein is analyzed by Spearman method,and the results show that there is a positive correlation between them(r = 0.249,P < 0.05).4.There is significant difference between the expression of LGALS1 protein in EOC and the pathological grade and clinical stage of EOC:when the pathology is high grade,the expression rate of LGALS1 protein is significantly increased,and when the clinical stage is late,the expression rate of LGALS1 protein is significantly increased(P < 0.05).There is no significant difference between the expression of LGALS1 protein in EOC and the age of onset,pathological type,serum CA125 and HE4 levels in patients with EOC.Conclusion:1.The expression levels of LGALS1 m RNA and protein are significantly increased in EOC tissues,which are closely related to the occurrence and development of EOC.2.The shortening of OS and PFS in patients with ovarian cancer with high expression of LGALS1 m RNA is a risk factor for OS and PFS in patients with EOC.3.The positive expression of LGALS1 protein is related to the pathological grade and clinical stage of EOC patients,and participates in the process of invasion and metastasis of EOC.4.The expression of FN1 protein is significantly increased in EOC tissues.There is a positive correlation between LGALS1 protein and FN1 protein expression in EOC tissues.LGALS1 protein may promote the occurrence and development of EOC by mediating the process of EMT.