Low Expression Levels Of The G0S2 Gene May Be Related To Acute Myocardial Infarction

Background and ObjectiveCoronary artery disease,and in particular coronary atherosclerotic disease(CAD),has an incidence of epidemic proportions,and has caused serious social and economic losses.It is estimated that the prevalence of cardiovascular disease will increase by approximately10% over the next 20 years.The current preferred biomarkers for diagnosis of acute myocardial infarction(AMI)are cardiac troponin I and T(Tn I and Tn T).However,especially in elderly patients,they will appear when they suffer from heart failure,chronic kidney disease or sepsis,false positive results of elevated Tn will occur.The G0 / G1 switch 2(G0S2)gene,encodes a small basic protein of103 amino acids,which is closely related to cell proliferation,lipolysis,apoptosis,oxidative phosphorylation,and various tumor diseases.Our research group previous gene chip study found that G0S2 gene expression in the peripheral blood leukocytes of AMI patients was lower than that of stable CAD(SCAD)patients.The aim of the present study was to expand the sample size to confirm the relationship between the expression of the G0S2 gene in peripheral blood and acute myocardial infarction(AMI)based on previous gene chip results,and whether G0S2 gene can be a feasible biomarker for AMI diagnosis.MethodsA total of 92 patients with AMI who were hospitalized in the Department of Cardiology in The Third Hospital of Jilin University from March to May 2018 were selected as the experimental group,and75 patients with SCAD were selected as the control group.Analyzed and compared the clinical data between the two groups,collected the peripheral blood leukocytes of the patients.mRNA expression levels of G0S2 in peripheral blood leukocytes was measured by real-time quantitative polymerase chain reaction(RT-PCR),and its protein expression levels determined by Western blot analysis.ResultsComparison of the clinical data analysis of the two groups of patients showed that in terms of gender,age,history of hypertension and smoking,type 2 diabetes,and levels of serum triglyceride(TG),serum total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),there was no significant difference between the two groups.Analysis of the expression level of G0S2 gene in peripheral blood leukocytes of AMI group and SCAD group: The relative expression of G0S2 gene mRNA and protein in peripheral blood leukocytes of AMI patients was significantly lower than that of SCAD patients.The relative mRNA expression of AMI group was 0.41-fold lower than that of the control group.The protein level expression was 0.45-fold lower than that of the control group.Correlation analysis between the relative expression of G0S2 gene and TG in peripheral blood leukocytes of all subjects showed that there was no significant correlation between the relative expression of G0S2 gene in peripheral blood leukocytes and TG.The binary logistic regression analysis of G0S2 gene mRNA expression level in peripheral blood leukocytes and AMI showed that G0S2 gene expression in peripheral blood leukocytes in the AMI group was lower than that in the SCAD group.The risk of AMI in the GOS2 gene low expression group in peripheral blood leukocytes was 2.04 times that of the high expression group.ConclusionG0S2 gene expression in the peripheral blood leukocytes of AMI patients was lower than that of SCAD patients.Low G0S2 gene expression in peripheral blood leukocytes is an independent risk factor for AMI in SCAD patients.The risk of AMI in the GOS2 gene low expression group in peripheral blood leukocytes was 2.04 times that of the high expression group.GOS2 gene may be a viable biomarker for the diagnosis of AMI.