Research On The Correlation Between Human Herpes Virus 6 And Female Infertility

Objective:Human Herpes Virus-6(HHV-6)is a more common virus,can be infected and replicated in human genital tract.To explore the relationship between HHV-6 and female infertility,so as to provide new ideas for the treatment of female infertility.Methods:Randomly select 75 infertility patients admitted to the outpatient and inpatient clinics of the Second Hospital of Jilin University from November 2019 to December 2020 as the experimental group,and randomly select 10 healthy fertile women as the control group during the same period.Take the endometrial tissue during hysteroscopy,extract and purify the RNA in the endometrial sample,reverse transcribed into c DNA,and use realtime quantitative PCR to detect he expression and relative expression of HHV-6 U94 and HHV-6 U42 in the endometrial tissue of the experimental group and the control group.SPSS 25.0 statistical software was used for analysis,using continuous correction method,P<0.05 was statistically significant.Results:1.The age of the experimental group was 32.73±3.81 years old,the duration of the patient’s menstrual period was 5.13±1.11 days,the menstrual cycle was 30.28±3.25 days,and the sample collection time was 12.20±1.05 days of menstruation.The age of the control group was 34.1±3.07 years,the duration of the menstrual period was 5.10±0.99 days,the menstrual cycle was 29.0±1.45 days,and the sample collection time was12.30±0.95 days of menstruation.There was no notable difference in age,menstrual period,menstrual cycle,and sample collection time between the two groups of patients(P>0.05);2.The expression of HHV-6 U94 was detected in 59 cases in the endometrium of the experimental group,and the expression rate of HHV-6 U94 was detected in 5 cases of endometrial tissue in the control group at 78.7%,and the expression rate was 50%,which was lower than the expression rate in the experimental group,and the difference was not statistically significant(P>0.05).However,the relative expression of HHV-6 U94 in the endometrium of the experimental group was 26.82±18.74,and the relative expression of HHV-6 U94 in the control group was 1.00±0.25.There was a statistically significant difference in the relative expression between the two groups(P<0.05).3.The expression of HHV-6 U42 was detected in 48 cases in the endometrium of the experimental group,the expression rate was 64%,and the expression of HHV-6 U42 was detected in 4 cases in the endometrial tissue of the control group,the expression rate was 40%,which was lower than the expression in the experimental group.The difference was not statistically significant(P>0.05).However,the relative expression of HHV-6 U42 in the endometrium of the experimental group was 20.43±17.31,and the relative expression of HHV-6 U42 in the endometrium of the control group was 1.00±0.42.There was a statistically significant difference in the relative expression between the two groups(P<0.05).4.Virus expression is defined as latent infection(expressing U94 alone)and active infection(expressing U94 and U42).Active infection was detected in 48 cases(64%)in the endometrium of the experimental group,and active infection was detected in 4 cases(40%)in the endometrium of the control group.The experimental group was higher than the control group,but the difference was not statistically significant(P>0.05).Latent infection was detected in 11 cases(14.7%)of the endometrium in the experimental group,and 1 case(10%)in the endometrium of the control group was detected with latent infection.The experimental group was higher than the control group,and the difference was not statistically significant(P>0.05).Conclusion:1.HHV-6 has a high infection rate in the endometrium.2.HHV-6 expression in the endometrium is associated with female infertility,but the specific mechanism of HHV-6 leads to female infertility needs to be further studied.