| Objective:1.The characteristics of plasma amino acid metabolism in patients with CHD were analyzed by targeted metabolomics.2.To further understand whether the change of amino acid level is an independent risk factor for coronary heart disease.3.To analyze whether the level of amino acids in patients with coronary heart disease is correlated with the degree of coronary artery disease.Methods : A total of 490 subjects who underwent coronary angiography in the Department of Cardiology of the Second Hospital of Dalian Medical University were included in this study,including 350 patients with coronary heart disease and 140 healthy subjects without obvious coronary artery disease.The plasma samples of all subjects in this study were separated by chromatography and mass spectrometry using metabonomics ultra performance liquid chromatography mass spectrometry(UPLC-MS)detection technique.Results:1.Compared with the healthy control group,the average levels of gender(male),smoking history,history of hypertension,hypersensitive C-reactive protein,blood uric acid and plasma fibrinogen were significantly increased in the CHD group,while the levels of total cholesterol and low density lipoprotein were significantly decreased(P <0.05).There were no significant differences in drinking history,age,BMI,white blood cells,plasma D-dimer,high-density lipoprotein,triglyceride,glutamic-oxalacetic transaminase,glutamic-pyruvic transaminase,serum creatinine and urea nitrogen levels between the two groups(P > 0.05).2.Compared with healthy control group,the plasma levels of leucine,valine,phenylalanine,asparagine,proline and homocysteine in CHD group were significantly increased,while the plasma level of glycine was significantly decreased(P < 0.05).There were no significant differences in 16 metabolic indexes such as residual alanine,serine,threonine,tryptophan and arginine between the two groups(P > 0.05).3.Leucine,valine,phenylalanine,homocysteine,glycine,asparagine,proline index into the multivariate Logistic regression model to analyze many factors,the results show that homocysteine and valine are independent risk factors for coronary heart disease incidence(OR values were 1.010,1.440),glycine is to protect the influence factors of coronary heart disease occurs(OR a value of 0.993),statistically significant difference(P< 0.05).However,proline,leucine,phenylalanine and asparagine were not included in the equation,suggesting that they were risk factors for coronary heart disease,but not independent risk factors.4.ROC curve was used to analyze the diagnostic value of homocysteine,glycine and valine levels in patients with CHD.The results showed that Hcy(AUC = 0.638)> Val(AUC = 0.580)> Gly(AUC = 0.575),suggesting that homocysteine had the greatest value in predicting the incidence of CHD.5.Pearson correlation analysis showed that plasma homcysteine and valine levels in the CHD group were positively correlated with Gensini score(correlation coefficient r was 0.479 and 0.315,respectively),while plasma glycine levels were negatively correlated with Gensini score(correlation coefficient r was-0.325),the difference being statistically significant(P < 0.05).Conclusion: 1.Plasma levels of proline,valine,leucine,homocysteine,phenylalanine and asparagine were positively correlated with the incidence of CHD,while plasma levels of glycine were negatively correlated with the incidence of CHD.2.High plasma levels of homocysteine and valine were independent risk factors for coronary heart disease,while low plasma levels of glycine were independent risk factors for coronary heart disease.3.The levels of glycine,valine and homocysteine in plasma have reference value for the diagnosis of CHD,and valine has the most reference value.4.Plasma levels of valine and homocysteine were positively correlated with the severity of coronary artery disease,while plasma levels of glycine were negatively correlated with the severity of coronary artery disease. |
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