Metabonomics Research On Patient Plasma With Phlegm Congestion Syndrome Type And Qi Deficiency Syndrome Type Coronary Heart Disease

Objective1. To investigate the distribution of coronary heart disease (CHD) major TCM (Traditional Chinese Medicine) syndrome type, and to analyze the relationship among the major TCM syndrome type, the results of coronary angiography and the blood lipids.2. Combining modern systems biology theory with TCM traditional methods, we make use of metabonomics technology platform based on GC/MS to carry out the research to the biochemical differentiation of Phlegm Congestion Syndrome Type and Qi Deficiency Syndrome Type of coronary heart disease.Method:1.166patients with CHD who had undergone CAG examination were categorized to several groups according to TCM syndrome. At the same time, the blood lipids level of the patients was tested. Correlating characteristics among the TCM syndromes, degree of coronary stenosis, number of stenosed vessels and blood lipids were analyzed.2. Selected patients with CHD of Phlegm Congestion Syndrome Type and Qi Deficiency Syndrome Type, used GC/MS techniques for the determination of the endogenous metabolites in the plasma of these two type, then analysised their metabolic spectrum on chemometrics pattern recognition method (MCTree). Result:1.101patients with coronary heart disease were blood stagnation type, accounting for60.84%,37cases of phlegm congestion type, which accounts for22.29%,28cases of qi deficiency type, accounting for16.87%. For the result of coronary angiography, the blood stagnation type and the phlegm congestion type had3major stenosed coronary vessels, which was a larger number than the stenosed coronary vessel number of qi deficiency type patients. The level of lesion was severe; Triglyceride (TG) levels in phlegm congestion type group was significantly higher than other groups(P<0.05), while total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL), apolipoprotein A1(ApoA1), apolipoprotein B (ApoB) levels of the group was no significant difference (P>0.05).2. Matching and identifying the total ion current with mass spectral library, and finally we got46kinds of accurate qualitative conpounds. The consequence of MCTree showed the differentiation that of phlegm group and Qi deficiency group. At the same time, it showed the classified contribution value of46kinds of substance, in which we could find the potential biomarkers. Such as serine, valine,2hydroxy propionic acid, peanut four oleic acid methyl ester, cholesterol, fructose, lactic acid. Conclusion:1. The results of coronary angiography and blood lipid levels have some sort of relativities with TCM syndrome, which can be used as a reference to TCM syndrome.2. The differences on metabonomics of CHD TCM syndrome types provide the reference of objective index for syndrome differentiation of TCM in CHD, prompt metabonomics technology would become a new research tools on TCM syndrome types study.