Study On The Anti-tumor Active Components Of Goniothalamus Yunnanensis

To provide scientific basis for further development and utilization of Goniothalamus yunnanensis,anti-tumor chemical constituents of stems and leaves of Goniothalamus yunnanensis were investigated.Goniothalamus yunnanensis was refluxed with 95%ethanol to get the ethanol extract,which was partioned with petroleum ether and ethyl acetate.The compounds were isolated and purified from ethyl acetate part by silica gel column chromatography,semi-preparative liquid chromatography,ODS chromatography and Sephadex LH-20 chromatography,and their structures were identified by ¹H-NMR,¹³C-NMR,UV,IR and MS mothods.Twenty-four compounds were isolated and identified,and their sructures type involved in alkaloids,styrenelactones,flavonoids,triterpenoids and steroids.The chemical constituents of Goniothalamus yunnanensis were studied and 24compounds were isolated and identified as aristolactam AII（1）,enterocarpam-II（2）,aristolactam AIIIa（3）,aristolactam BII（4）,griffithinam（5）,piperolactam A（6）,lysicamine（7）,N-p-Coumaroyltyramine（8）,marcanines B（9）,9-deoxygoniopypyrone（10）,goniofufurone（11）,goniothalamin（12）,pinocembrin（13）,β-sitosterol（14）,palmitic acid（15）,lauryl alcohol（16）,triacontanol（17）,inearnatyl alcohol（18）,glycerol monopalmitate（19）,pureonebio（20）,diethyl disulfoxide（21）,stigmasterol-3-O-β-D-glycopyranoside（22）,cheliensisamine（23）,and ursolic acid（24）.Compounds1–24 were isolated from Goniothalamus yunnanensis for the first time.The antitumor activities of the compounds were tested by SRB mothod,and among them,the inhibitory activities of 13 compounds on HCC1806 cells,HCT116 cells and Hela cells at different concentrations were recorded.The compounds in Goniothalamus yunnanensis showed moderate inhibitory effects on triple negative breast cancer HCC1806,colorectal cancer HCT116 and cervical cancer Hela.the inhibition rates of compounds 7,12 and 13 on triple negative breast cancer HCC1806 were 95.31%,77.73%and 58.64%when the concentration at 50μM,respectively;and the inhibition rates of compounds 7 and 12 on colorectal cancer HCT116 were 98.21%and 86.89%,respectively.The inhibition rates of compounds 7 and 12 on Hela tumor were 96.71%and 76.95%,respectively.The structure types and pharmacological activities of Annonaceous acelogenins compounds were summaried.Structure types of Annonaceous acelogenins included the Iso adjacent bis-THF ACGs,Mono-THF ACGs,Adjacent bis-THF ACGs,and Nonadjacent bis-THF ACGs.Furthermore,the compounds demonstrated good physiological and pharmacological activities in the aspects of insecticidal,antimicrobial and tumor cell growth inhibition.