Study On The Chemical Composition And Polysaccharide Activity Of By-products In The Preparation Of Zucamu Granules

Objective: To provide the basis for researching the development and utilization of the active ingredients in the by-products in the preparation of Zucamu Granules,this research study the chemical composition and the Immunity and antioxidant activity of polysaccharides in the by-products in the preparation of Zucamu Granules.Methods: The by-products of Zucamu Granules were extracted by distilled water and ethanol,and the chemical components were identified by chemical composition pre-experiment;the contents of 5 kinds of total chemical components in the by-products of Zucamu Granules were detected by the UV-Vis Spectrophotometry;the contents of aloe emodin,emodin and Chrysophanol in the by-products of Zucamu Granules were measured by HPLC;the decolorization and purification process of the by-product polysaccharides of Zucamu Granules was optimized by macroporous adsorption resin method;the effects of the by-product polysaccharides of Zucamu Granules on the proliferation,phagocytosis and NO secretion of RAW264.7 cells were analyzed;and the chemical and cellular antioxidant activities of the by-product polysaccharide of Zucamu Granules were investigated.Results: the results of chemical composition pre-experiment showed that the by-products of Zucamu Granules contained 7kinds of components,such as polysaccharide,amino acid,polyphenol,etc;the contents of polysaccharide,polyphenol,amino acid,protein and flavone in the by-products of Zucamu Granules were 245.2-308.2,79.70-92.32,28.20-32.47,24.90-25.96,0.2719-0.3119 mg/g separately.The content of aloe-emodin,emodin and Chrysophanol in the by-product was 0.0822-0.0948,0.0578-0.1053 and 0.5917-0.9510 mg/g respectively.The best purification condition of the polysaccharide of by-product are:AB-8 macroporous adsorption resin,sample concentration 2.30 mg/m L,sample amount 1.85 BV,elution velocity 1.50 m L/min.The polysaccharides in the by-product can promote the proliferation,phagocytosis and no secretion of RAW264.7 cells,and ha antioxidant activity in vitro.Conclusions: the chemical composition of the by-product of Zucamu Granules was analyzed qualitatively and quantitatively.It was found that the polysaccharide of the by-product of Zucamu Granules had certain immune and antioxidant activities,which laid a foundation for the green development of the by-product of Zucamu Granules.