| Objective To investigate the concentration of serum microfibrillar-associated protein 4(MFAP4)and Fibulin-1(FBLN-1)in patients with bronchial asthma,and to explore the expression level and clinical significance of MFAP4 and FBLN-1 in bronchial asthma,so as to provide guidance for the diagnosis and treatment of bronchial asthma.Methods70 patients with bronchial asthma were collected,including 35 patients with asthma whose serum total Ig E > 100IU/ml(Ig E elevated asthma group),and 35 patients with asthma whose serum total Ig E ≤ 100 IU/ml(Ig E normal asthma group).All the above patients were treated in the Department of Respiratory Medicine ward and outpatient of the Second Affiliated Hospital of Shenyang Medical College from December 2019 to December 2020.35 cases of healthy physical examination in the same period were collected(control group).The concentrations of serum MFAP4 and FBLN-1 in these three groups were detected by enzyme linked immunosorbent assay(ELISA),and the general clinical data of all subjects were recorded.SPSS 25.0statistical software was used to analysis the general clinical data,serum MFAP4 and FBLN-1 levels of the subjects in each group.The test level was α = 0.05,and P<0.05 indicated that the difference was statistically significant.The Receiver operating characteristic curve(ROC)was drawn,and the area under the curve(AUC),sensitivity,specificity and Yoden index were calculated respectively.Combined with ROC curve, the predictive value of serum MFAP4 and FBLN-1 in asthma was evaluated,and the cut-off point was determined.Results(1)There was no significant difference in the general clinical data(sex,age,smoking history and so on)among the three groups(P>0.05).(2)The serum MFAP4 concentration in the Ig E elevated asthma group,the Ig E normal asthma group and the control group was 1072.84±306.93 pg/ml,882.21±159.11 pg/ml,and777.16±121.01 pg/ml,respectively.The mean values was not equal among the three groups.The serum MFAP4 concentration in the Ig E elevated asthma group and Ig E normal asthma group were significantly higher than that in the control group,and the serum MFAP4 concentration in the Ig E elevated asthma group was significantly higher than that in the Ig E normal asthma group(P<0.05).(3)The serum FBLN-1concentration in the Ig E elevated asthma group,the Ig E normal asthma group and the control group was 356.90±76.04 pg/ml,259.55±34.97 pg/ml,and 217.11±50.03 pg/ml,respectively.The mean values was not equal among the three groups.The serum FBLN-1 concentration in the Ig E elevated asthma group and Ig E normal asthma group were significantly higher than that in the control group,and the serum FBLN-1concentration in the Ig E elevated asthma group was significantly higher than that in the Ig E normal asthma group(P<0.05).(4)The AUC of serum MFAP4 in predicting asthma was 0.762(95%CI: 0.674-0.850,P<0.05),the sensitivity was 0.557,the specificity was 0.914,the Yoden index was 0.471,and the optimal cut-off point was917.25 pg/ml.(5)The AUC of serum FBLN-1 in predicting asthma was 0.861(95%CI:0.785-0.937,P<0.05),the sensitivity was 0.9,the specificity was 0.686,the Yoden index was 0.586,and the optimal cut-off point was 230.86pg/ml.(6)The AUC of serum MFAP4 and FBLN-1 combined in predicting asthma was 0.887(95%CI: 0.821-0.954,P<0.05),the sensitivity was 0.914,the specificity was 0.743,and the Yoden index was0.657.Conclusions 1.The expression level of serum MFAP4 and FBLN-1 was significantly increased in patients with asthma.The expression level of MFAP4 and FBLN-1 in serum of asthma patients with elevated total Ig E was higher than that of asthma patients with normal Ig E.2.The increase of serum MFAP4 and FBLN-1 can predict the occurrence of asthma to some extent. |
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