Study On The Mechanism Of Tuhuaidan Siwu Decoction In The Treatment Of Psoriasis Vulgaris

Objective:psoriasis is one of the most common chronic inflammatory skin diseases.It is an immune-mediated chronic inflammatory hereditary skin disease.The occurrence of the disease has the characteristics of long course,difficult to cure quickly and easy to relapse,which has a serious impact on the body,psychology and spirit of the patients.The underlying pathological mechanism involves the complex interaction between innate immune system and adaptive immune system.It affects up to 5% of patients worldwide.Western medicine can not be used for a long time due to the toxic and side effects of drugs,and tuhuaidan Siwu Decoction has a good clinical effect in the treatment of psoriasis vulgaris,but the mechanism of its action in the treatment of psoriasis is still unclear.In this paper,network pharmacology is used to provide reference for experimental research,and then through the combination of experimental research and statistical analysis,the effects of tuhuaidan Siwu Decoction on imiquimod induced psoriasis mice and lipopolysaccharide induced Ha Ca T cells in vitro model of psoriasis are explored.Methods: this paper is divided into three parts.1.Based on network pharmacology,the potential active ingredients,targets,signaling pathways and biological processes of Smilax glabra in the treatment of psoriasis vulgaris were analyzed to find the related targets and important biological metabolic pathways for the treatment of psoriasis vulgaris,so as to provide modern reference for the follow-up experimental research.2.Establish the back skin psoriasis like animal model of mice induced by imiquimod,give different concentrations of tuhuaidan Siwu Decoction by gavage,prepare the drug containing serum,through the detection of drug containing serum to explore the mechanism of tuhuaidan Siwu Decoction in the treatment of psoriasis vulgaris.3.To explore the mechanism of tuhuaidan Siwu Decoction in the treatment of psoriasis vulgaris,we established the psoriasis cell model in vitro,gave different concentrations of tuhuaidan Siwu Decoction,detected the cell survival rate of each group by CCK-8 method to screen the best dosage and detected the supernatant of experimental cells in each group.Results:1.Through the network pharmacology research,12 active ingredients related to psoriasis were screened from Smilax glabra,and 107 targets and 7 metabolic pathways were intervened to play a role in the treatment of psoriasis.2.2.Compared with normal mice,the expressions of IL-6,AKT1 and TNF-α in serum of model group were up-regulated.Compared with blank group,the contents of TNF-α,IL-6 and AKT1 in model group were up-regulated(0.270 ± 0.003),(24.99 ± 0.747),(9.301 ± 0.363),respectively(P < 0.001).Compared with model group,the contents of TNF-α,IL-6 and AKT1 in low dose group and medium dose group of tuhuaidan Siwu Decoction were up-regulated The contents of IL-6,AKT1 and TNF-α in group A and high-dose group of tuhuaidan Siwu Decoction were significantly decreased,especially in high-dose group of tuhuaidan Siwu Decoction(0.125 ± 0.004),(13.1 ± 0.706),(7.982 ± 0.186)(P < 0.001).Compared with the normal group,the expression of IL-6,AKT1 and TNF-α in the cell supernatant of the model group was also up-regulated.Compared with the model group,the levels of IL-6,AKT1 and TNF-α in the serum and IL-6,AKT1 and TNF-αin the cell supernatant of the treatment group were decreased,Compared with the blank group,the contents of TNF-α,IL-6 and AKT1 in the model group were increased(1.202± 0.125)(P < 0.0001),(48.17 ± 1.991)(P < 0.001),(9.386 ± 0.352)(P < 0.001),respectively;compared with the model group,the contents of TNF-α,IL-6 and AKT1 in the low-dose group,middle dose group and high-dose group were decreased,especially in the high-dose group,061)(P < 0.001),(27.85 ± 1.329)(P < 0.001),(7.939 ± 0.219)(P< 0.0001).Conclusion: psoriasis can up regulate the expression of IL-6,AKT1,TNF-αand other inflammatory factors,while tuhuaidan Siwu Decoction can down regulate the high expression of inflammatory factors.It shows that tuhuaidan Siwu Decoction can treat psoriasis by reducing the expression of inflammatory factors in serum.