Studies On The Secondary Metabolites Of Spondias Pinnata And Coptosapelta Diffusa

Medicinal and edible plants play an important role in disease prevention and treatment.The extracts of Spondias pinnata(L.f.)Kurz fruits and the aerial parts of Coptosapelta diffusa(Champ.Ex Benth.)Steenis have significant anti-virulence and antibacterial activities.The chemical constituents of the two plants were separated and purified by column chromatography of silica gel,RP-1 8,Sephadex LH-20 and HPLC 38 compounds were obtained and their structures were identified by NMR,MS,UV and IR spectroscopy techniques.It provides a theoretical basis and material basis for the rational development and utilization of plantsFrom the ethanol extract of S.pinnata fruits,15 known compounds were elucidated including 5 caffeoylquinic acid derivatives,5-O-caffeoylquinic acid methyl ester(B1),5-O-caffeoylquinic acid(B2),5-O-caffeoylquinic acid ethyl ester(B3),3-O-caffeoylquinic acid methyl ester(B4)and 3-O-caffeoylquinic acid n-butyl ester(B5);3 coumarin,grevillone(B6),isoscopoletin(B7)and isoscopolin(B8);2 flavonoid glucosides,isoquercitrin(B9)and rutin(B10);3 organic acids,gallincin(B11),dimethyl malate(B12)and trimethyl ester of hydroxycitric(B13);one aromatic glycoside,1-p-Coumaroyl-β-D-glucose(B14)and one adenosine(B15).Caffeoylquinic acid derivatives were isolated from this fruit for the first time,and 5-O-caffeoylquinic acid methyl ester(B1)was the main component with the content of 258.38 mg/kg.Compounds B1-B15 did not show remarkable antibacterial and anti-13 SS virulence activities.From the ethanol extract of the aerial parts of C.diffusa,23 compounds were elucidated,including 6 iridoid glucosides,namely coptosapides A-F(H1-H6),3 guaiane-type sesquiterpenoids,namely diffuseterpenoids A-C(E1-E3)and one tetrahydroanthraquinone,namely diffusequinones D(E4),together with 7 anthraquinones,2-Methylanthraquinone(E5),2-Methoxyanthraquinone(E6),2-Formylanthraquinone(E7),2-Hydroxyanthraquinone(E8),2-Hydroxymethyl anthraquinone(E9),7-Hydroxy-2-methylanthraquinone(E10),2-Hydroxy-6-hydroxymethyl-anthraquinone(E11);4 coumarin,Isoscopoletin(E12),Isofraxidin(E13),6,7,8-Trimethoxycoumarin(E14),Isoscopolin(E15);2 aromatic compounds,Methyl 1,4-dimethoxy-2-naphthoate(E16)and Guaiacylglycerol(E17).Compounds E4,E7 and E8 showed significant antibacterial activity against Staphylococcus aureus ATCC25923,with MIC of 8,4 and 8 μg/mL,respectively.