Preparation,Physicochemical Properties And Co-culture Of 3D Printed Bionic Coral Artificial Bone

Objective: 1.To study the preparation of 3d printed bionic coral artificial bone materials.2.Analyze the physical and chemical properties of 3d printed bionic coral artificial bone,and verify that it is a good substitute material for bone defect transplantation.3.To study the effects of co-culture,toxicity,adhesion and osteogenesis of 3d printed biomimetic coral artificial bone materials and cells,and to provide a research basis for animal experiments and clinical trials of 3d printed biomimetic artificial bone materials.Methods: 3D bio-printer was used to print polylactic acid,calcium carbonate and hydroxyapatite.Stress-strain experiment of three-dimensional printing bionic artificial bone material;XRD was used to observe the composition of inorganic materials in the 3d printed biomimetic coral artificial bone materials.SEM was used to observe the surface structure,micropores and pores of the 3d printed biomimetic artificial bone material.EDS was used to determine the element composition of 3d printed bionic coral artificial bone material.The content of carbonate and phosphate in three dimensional biomimetic coral artificial bone was determined by FTIR.Cck-8from co-cell culture of MC 3T3 and biomimetic coral artificial bone material was used to determine cell proliferation and toxicity of the material.3d printed biomimetic coral artificial bone material and MC 3T3 co-cell culture were used to observe the adhesion of actin filaments by confocal laser microscopy for 1,4 and 7 days.3 d printed biomimetic coral artificial bone material and MC 3T3 co-cultured cells were cultured for 14 days.Results: the bionic coral artificial bone material was printed by 3d biological printer,with the size of 1cm X1 cm X1cm.Stress-strain test of 3d printed bionic coral artificial bone material: ultimate compressive strength: 15.03 mpa,elastic modulus:20.51 gpa.The content ratio of inorganic calcium carbonate and hydroxyapatite was determined by XRD.The appearance of the 3d printed bionic coral artificial bone material was observed by scanning SEM electron microscope.The pore diameter of the material was present,uniform in size,and the pore passage was extended inside.The pore size was 600～800um,and the structure and pore wall were intact.The ratios of elements were determined by EDS,C: 19.66%,N: 2.78%,O: 54.42%,P: 2.04%,and ca21.10%.The content ratio of carbonate and phosphate was determined by FTIR.Cck-8 assay of 3d printed biomimetic coral artificial bone material and MC 3T3co-cell culture for 1,4 and 7 days showed statistical difference between the experimental group and the control group(P<0.05),and the experimental group was superior to the control group.The 3d printed biomimetic coral artificial bone material and MC 3T3 co-cell culture were observed by confocal laser microscopy on day 1,4,and 7.On day 7,actin microfilaments were observed by comparison with day 1.There was a statistical difference between the experimental group and the control group(P<0.05),and the experimental group was superior to the control group.Conclusion: 1.Preparation of 3d printed bionic coral artificial bone materials.2.The mechanical properties,porosity and material composition of the 3d printed bionic coral artificial bone material conform to the physical and chemical properties of the bone defect implant material.3.3d printed bionic coral artificial bone material co-cultured with cells can promote the proliferation,adhesion and osteogenesis of bone cells.