Experimental Study Of Non-invasive Imaging In Staging Of Deep Venous Thrombosis

PART one The value of ultrasound shear wave elastography in the staging diagnosis of femoral venous thrombosis in rabbitsObjectiveTo study the feasibility of ultrasound shear wave elastography in the staging diagnosis of femoral venous thrombosis in rabbits,whether thrombus recanalization affects the measurement results or not.MethodsThe model of rabbit femoral vein thrombosis was established,Young’s Modulus value(YM)of thrombus body was measured continuously for 16 days using ultrasound shear wave elastography(SWE)technology,In the case of thrombosis recanalization,non-recanalization site(stable site)and recanalization site were measured respectively,and the difference in YM between recanalization site and stable site of all rabbits on day16 was compared.Two rabbits were randomly selected for HE pathological sections from the body tissues of thrombus every day,and pathological changes were observed under the microscope.Results1.From day 1 to day 7,YM showed a slow rising trend with a similar rate.From day 7 to day 13,the change range was sharp and the rate varies,among which the rate was the fastest from day 8 to day 9,and the YM tends to be stable after the day 13.The results of repeated measurement anova showed that the YM had a linear trend between day 1,day 3,day 8 and day 16,and the difference was significant between any two days(P<0.05).2.On day 12,two experimental rabbits showed recanalization of the thrombus body.At the recanalization site,and the color doppler ultrasound could detect the point and strip blood flow signal,some of the spectrum could be measured.On day 16,the YM at the recanalization site of the thrombus body was significantly decreased,which was significantly different from that at the stable site(P<0.05).3.Pathological sections of thrombus showed recanalization in the peripheral area of thrombus body and reappearance of erythrocyte aggregation on day 11,the transformation of thrombus from acute phase to chronic phase was completed in 16 days.ConclusionUltrasound shear wave elastography could show the changes of elastic hardness of femoral vein thrombosis quantitatively in rabbits,and it was expected to be an effective quantitative index to evaluate the stages of venous thrombosis.The YM at the recanalization site was significantly decreased by thrombus recanalization.PART two Application value of 3D-TSE sequence in staging of femoral venous thrombosis in rabbitsObjectiveTo explore the application value of 3d-tse sequence in staging of rabbit femoral venous thrombosis.MethodsFemoral vein insufficiency ligation and limb fixation were used for modeling,9 successfully modeled rabbits were divided into 1-day group,7-day group and 14-day group according to the completely random method.3 rabbits in each group.After the examination of each group,the thrombus body tissues were taken to make HE pathological sections,and pathological lesions were observed under the microscope,which was used as the gold standard to verify the above detection.ResultsOne died during the 1-day group,and no significant thrombus was observed in the two experimental rabbits in the 14-day group.On T1WI and T2WI,both the 1-day group and the 7-day group showed the main contour signal,and the thrombosis morphology,size and signal of the two groups showed little change.In the 14-day group,T1WI and T2WI showed low signal,and compared with the first two groups,the shape,size and signal changed greatly.In all three groups,not only thrombus changes,but also edema of peripheral muscle tissue were observed.ConclusionMR 3d-tse sequence can show the changes in acute and chronic stages of thrombosis,which is feasible in the staging of rabbit femoral vein thrombosis.