Chemical Constituents Of Bai Nationality Medicine Rubus Delavayi And Nothopanax Delavayi

Objective:The present study aims to study the chemical constituents of R.delavayi and N.delavayi.And to predicte the action targets for anti-Trichinella spiralis bioactive components of R.delavayi and the action targets for anti-dental ulcer bioactive components of N.delavayi.Methods:The compounds were isolated and purified by various column chromatography methods including silica gel,Polyamide,Sephadex LH-20,MCI and preparative HPLC column chromatography.Their structures were identified based on spectral analysis.Predicting the action targets for anti-Trichinella spiralis bioactive components of R.delavayi and the action targets for anti-dental ulcer bioactive components of N.delavayi based on network pharmacology.Results:Twenty-seven compounds were obtained from water extract of R.delavayi,and identified as negunfurolside(1),schensianoside A(2),amarantholidoside V(3),(3R,6E,10R)-11-β-D-glucopyranosyloxy-3,10-dihydroxy-3,7,11-trimethyldodeca-1,6-diene(4),5-meth oxyresorcinol(5),methyl 3,5-dihydroxyphenylacetate(6),catechol(7),salicylic acid(8),gallic acid(9),l-β-D-glucopyranosyloxy-3-methoxy-5-hydroxybenzene(10),taxicatin(11),benzyl-O-β-D-glucopyranoside(12),3,5-dihydroxyphenyl 1-O-(6’-O-trans-feruloyl)-β-D-glucopyranoside(13),trnas-1-O-methyl-6-O-caffeoyl-β-D-glucopyranose(14),(1’S,2’R)-guaiacyl glycerol(15),(1’R,2’R)-guaiacyl glycerol(16),teasperol(17),(+)-catechin(18),(-)-epi-catechin(19),kaempferol-3-O-β-D-glucoside(20),kaempferol-3-β-D-(6-O-cis-p-coumaroyl)glucopyranoside(21),kaempferol 3-O-α-L-rhamnopyranosyl(1→6)-β-D-glucopyranoside(22),bridelionol B(23),arjunglucoside I(24),glucosyl tormentate(25),β-sitosterol(26),daucosterol(27).A total of three targets and thirty-seven related pathways related to the chemical constituents of N.delavayi and Trichinellosis were selected out through database analysis.Eight compounds were obtained from Et OAc-soluble part of 80% ethanol extract of stem and leaves of N.delavayi,and identified as liquidambaric lactone(1),serratagenic acid(2),syringaresinol(3),3-oxo-6β-hydroxylean-12-en-28-oic acid(4),3β,29-dihydroxy-olean-12-en-28-oic acid 28-O-β-D-glucopyranosyl ester(5),5-O-caffeoylquinic acid methyl ester(6),β-sitosterol(7),carotene(8).A total of five targets and ninety-six related pathways related to the chemical constituents of N.delavayi and Trichinellosis were selected out through database analysis.Conclusion:1.Twenty-seven compounds including four sesquiterpenoids,eight phenolic acids,four phenylpropanoids,six flavonoids,two triterpenes,two steroids and one others.Compounds 4-8,10-13,15-17,20,23-25 were isolated from R.delavayi for the first time.Phenolic acids and sesquiterpenoids are the main constituents of R.delavayi.The results of network pharmacology analysis showed that: The chemical constituents of R.delavayi can regulate the expression of three Trichinellosis-related genes,MMP9,SDS and BCHE.The results of pathway enrichment analysis showed that most of the genes were enriched to MAPK signaling pathway,progesterone-mediated oocyte maturation pathway and TNF signaling pathway.However,these mechanisms of action still need further experimental verification.2.A total of eight compounds including four triterpenes,two steroids,one lignan,and one phenylpropanoid were isolated and identified from stems and leaves of N.delavayi.Five compounds(1-2,4-6)were isolated from genus Nothopanax for the first time.The results of network pharmacology analysis showed that: The chemical constituents of N.delavayi can regulate the expression of three Trichinellosis-related genes,AKR1B1,AKR1B10,SLC37A4,SLC6A4 and PDE4 D.The results of pathway enrichment analysis showed that most of the genes were enriched to Inflammatory mediator regulation of TRP channels and HIF-1 signaling pathway.However,these mechanisms of action still need further experimental verification.