Primary Investigation Into Overexpression Of Nucleolar Spindle Protein 1(NuSAP1) Gene Promotes Malignant Biological Behavior Of Ovarian Cancer And Possible Mechanism

Objective To screen gene chip with bioinformatics,looking for differentially expressed molecules between ovarian cancer and normal ovarian tissue,seeking out Nu SAP1 gene for the experimental study.In vitro experiments,to study effects of knocking down of Nu SAP1 gene on the cell phenotype,like cell proliferation activity,metastatic ability and cell cycle of ovarian cancer.and explore possible molecular mechanisms.Method Bioinformatics method was utilized to select gene chip from public network platform,which screened out the differentially expressed molecules between ovarian cancer and normal ovarian tissues.Si RNA was used to knock down Nu SAP1 gene by liposome-mediated transfection method,knock efficiency was tested with polymerase chain reaction and western blot.After silencing Nu SAP1,the proliferation of ovarian cancer cells was detected by CCK8 assay and clonogenic assay,and the migration ability of ovarian cancer cells was verified by scratch test,besides,the impact on cell cycle was detected by flow cytometry.Finally,the effects of Nu SAP1 knockdown on cyclin and Wnt / β-catenin signaling pathways were examined by polymerase chain reaction and western blot.Result After gene chip analysis,we screened CDKN2 A,EHF and NUSAP1 as candidate factors,and further verified the result with q PCR experiments in clinical ovarian cancer samples.Knockdown Nu SAP gene with transfection si RNA via liposome can significantly inhibit SKOV-3cells in proliferation,migration,and induce cell cycle arrest as well.Further exploration revealed that abnormal expression of Nu SAP1 may exert its function through the Wnt / β-catenin signaling pathway.Conclusion The abnormally high expression of Nu SAP1 in ovarian cancer may promote the malignant biological behavior of ovarian epithelial carcinoma,which may be brought about through Wnt pathway regulating pathway by EMT.