| Objective: the health burden of cancer is increasing in China,with more than 1.6 million people diagnosed and 1.2 million dying of cancer each year.As in most other countries,breast cancer is now the most common cancer among Chinese women;China accounts for 12.2 percent of new breast cancer diagnoses worldwide and 9.6 percent of deaths from the disease.Current projections and statistics indicate that the incidence and associated mortality of breast cancer is on the rise globally.The global incidence of breast cancer in women is projected to reach about 3.2 million new cases per year by 2050.Among them,triple-negative breast cancer(TNBC)has the characteristics of high metastasis and high recurrence,poor prognosis,and the expected survival time is still not satisfactory.These figures reflect the size of the incidence of breast cancer,its impact on societies around the world,and the urgency of prevention and treatment,which have yet to be achieved despite advances in clinical treatments such as surgery and chemotherapy.Therefore,the development of effective and safe early diagnosis and treatment strategies for breast cancer remains a global public health imperative.Nucleolar Spindle?Associated Protein(NuSAP1)is a recently discovered vertebrate protein with a molecular weight of about 55 kd,located on the chromosome arm,and involved in spindle assembly regulation and normal mitotic maintenance by binding to the microtubules and DeoxyriboNucleic Acid(DNA)domain.At present,many scholars have found that overexpression of NuSAP1 is closely related to a variety of malignant tumors.In this study,we analyzed the effect of NuSAP1 expression on the prognosis of breast cancer and explored the potential mechanism of NuSAP1 tumorigenesis in TNBC cells.Methods: 1.Correlation analysis of the expression level and clinical characteristics of NuSAP1 in breast cancer.RNA-seq breast cancer data was downloaded from the cancer genome atlas(TCGA),and the NuSAP1 gene was screened by R software.The R software was used to screen and analyze the clinical data of breast cancer patients,and the survival curve was drawn by Kaplan-Meier plotter.2.Real-time quantitative polymerase chain reaction(QRT-PCR)and Western blotting were used to detect the expression level of NuSAP1 in four breast cancer cells and one normal breast epithelial cell.3.Effects of NuSAP1 on TNBC biology.(1)lentivirus-mediated and plasmid transient transfection were used to silence or overexpress the expression of NuSAP1 gene in TNBC cells(BT-549,MDA-MB-231),a negative control group(NC)was set up,NuSAP1 group was knocked down(sh-NuSAP1),and the overexpressed NuSAP1 group(sh-NuSAP1 + ovNuSAP1)was knocked down,and the proliferation,migration and invasion ability of cells in each group was detected by CCK8 and Transwell experiments.The expression levels of proteins related to NuSAP1,Wnt/?-catenin and epithelial-mesenchymal Transition(EMT)were detected by QRT-PCR and Western blotting.Results: 1.The expression of NuSAP1 was up-regulated in breast cancer.Differential expression of NuSAP1 is associated with a variety of clinicopathological factors.The higher the expression level of NuSAP1,the shorter the survival time.2.The expression of NuSAP1 in breast cancer cells is higher than that in normal breast epithelial cells,especially in MDA-MB-231 and BT549 cells.3.The proliferation and invasion ability of sh-NuSAP1 group cells decreased;Expression of Cyclin D1,Vimentin,Slug,Twist,Wnt3 a and ?p-catenin decreased.E-cadherin expression was significantly increased.The shNuSAP1 + ov-NuSAP1 group showed the opposite result.Conclusion: NuSAP1 is an oncogene that affects the proliferation and invasion of TNBC through Wnt/?-catenin and EMT pathways. |
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