The Function And Mechanism Study Of MiR-589-5p In Hepatocellular Carcinoma

Background Hepatocellular carcinoma(HCC)is one of the most common tumors worldwide.Radical surgery remains the curative method of choice for HCC,due to the absence of effective marker for early diagnosis,considerable patients were diagnosed at late stage.micro RNAs used to be judged as “noise”,growing evidences have verified that micro RNAs play a vital role in epigenetics and post-transcription.The expression,function and mechanism of miR-589-5p in HCC are not well elucidated.In this investigation,we studied the role of miR-589-5p in the tumorigenesis of HCC.Methods we screened the potential miRNA in the pathological process of HCC by analyzing the data of The Cancer Genome Atlas(TCGA).We subsequently detected the expression level of miR-589-5p in HCC tissues and adjacent normal tissues with quantitative real-time PCR and then studied its clinical significance.The function of miR-589-5p on HCC was studied via loss of function in vitro and in vivo.The target gene was found by bioinformation analysis,luciferase reporter assay and function rescue assay were performed to verified the binding.Results miR-589-5p was significantly upregulated in HCC and higher expression of miR-589-5p was positively correlated with poorer prognosis,later stage and worse histological grade(P < 0.05).Functionally,suppression of miR-589-5p leaded to cell cycle arrest and apoptosis in vivo and in vitro.Depletion of miR-589-5p inhibited migration and invasion.Further study showed that miR-589-5p is involved in the EMT process of HCC.Mechanistically,we identified NFATc3,an important transcription factor of non-canonical Wnt signaling pathway,as one downstream target gene of miR-589-5p,which might suppress the progress of HCC.Conclusion Our findings revealed that miR-589-5p can promote the development of HCC via NFATc3 and may serve as a potential therapeutic target for HCC.