Mir-30a Inhibits The Epithelial-mesenchymal Transition Of Podocytes Through Downregulation Of NFATc3

MicroRNAs(miRNAs) possess an important regulating effect among numerous renal diseases, while their functions in the course of epithelial-to-mesenchymal transition(EMT) after podocyte injury remain unclear. The objective of our study is to identify the potential functions of miR-30 a in EMT of podocytes and explore the underlying mechanisms of miR-30 a in the impaired podocytes. The results revealed that downregulation of mi R-30 a in podocyte injury animal models and patients, highly induced the mesenchymal markers of EMT including Collagen I, Fibronectin and Snail. Furthermore, overexpression of miR-30 a enhanced epithelial markers(E-cadherin) but diminished mesenchymal markers(Collagen I, Fibronectin and Snail) in podocytes. In addition, we established miR-30 a target NFATc3, an important transcription factor of Non-canonical Wnt signaling pathway. More importantly, our findings demonstrated that the augmentation of miR-30 a level in podocytes inhibited the nuclear translocation of NFATc3 to protect cytoskeleton disorder or rearrangement. In summary, we uncovered the protective function of miR30 a targeting NFATc3 in the regulation of podocyte injury response to EMT.