The Combined Effect Of Astragaloside Iv And Carbon Nanoparticles On Fas/FasL Pathway In Gastric Ulcer

The occurrence of gastric ulcer is mainly relevant to the apoptosis of gastric mucosal epithelial cells.Clinical studies have shown that astragaloside Ⅳ and mongolian medicine can effectively treat gastric ulcer.The purpose of this study was to investigate the effects of mongolian medicine and its carbon nanoparticles on Fas/FasL apoptotic pathway-mediated gastric ulcer in mice,so as to confirm the pharmacodynamic components of mongolian medicine in the treatment of gastric ulcer,and lay the foundation for making an approach to the combined effects of astragaloside Ⅳ and carbon nanoparticles.Firstly,we established the damage model of gastric mucosal epithelial cell to investragate the effects of various drugs on the toxicity,expression of apoptosis-related genes in Fas/FasL apoptotic pathway and Caspase-3 protein activity in the model cells.Secondly,we studied the effects of various drugs on FasL expression in spleen T lymphocyte and the integrality of gastric mucosa of mice.Finally,we investigated the combined effects of astragaloside Ⅳ and carbon nanoparticles on the expression of Fas/FasL,expression of apoptosis-related genes in Fas/FasL apoptotic pathway and gastric mucosal integrity of mice.The results showed that:(1)From the studies in cells,we found that astragaloside Ⅳ and Mongolian medicine significantly regulate the expression of apoptosis-related genes Fas/Bax/BcL-2 and the activity of Caspase-3 in gastric mucosal cells induced by ethanol to achieve the recovery of inflammatory gastric mucosal cells.From the studies of three carbon nanoparticles,we found that graphene oxide and carboxylated single-walled carbon nanotubes,except fullerenes,had restorative effect on gastric mucosal cells induced by ethanol.(2)From the studies in animals,we found that mongolian medicine could significantly down-regulated the expression of FasL in spleen T lymphocyte of mice.At the same time,the results of tissue sections also showed that mongolian medicine had good protective function on gastric mucosa tissue of mice.From the studies of three sorts of carbon nanoparticles,we found that graphene oxide had the same function as mongolian medicine;carboxylated single-walled carbon nanotubes had the opposite effect with mongolian medicine,which had no protective effect on gastric mucosal tissue,but also might accelerate the damage of gastric mucosal tissue;fullerene had little effect.These results indicated that graphene oxide may play a major role in the treatment of gastric ulcer by mongolian medicine.(3)From the studies on the combined effect of astragaloside Ⅳ and carbon nanoparticles,we founded that astragaloside Ⅳ combined with graphene oxide could significantly reduce the expression of Fas protein in gastric mucosal epithelial cells induced by ethanol,and could significantly regulate its apoptosis-related genes Fas,Bax,BcL-2 and Caspase-3 activity.However,there was no significant difference in the expression of Fas protein,while combination with fullerens or carboxylated single-walled carbon nanotubes.From the studies in animals,we founded that astragaloside Ⅳ combined with graphene oxide could significantly down-regulate the expression of FasL in spleen T lymphocytes of mice,and protected the gastric mucosa of mice much better.In summary,Mongolian medicine can treat the gastric ulcer of mice by affecting the Fas/FasL apoptotic pathway,and the effect of graphene oxide is most significant among the three carbon nanoparticle components.Compared with the single action of astragaloside or graphene oxide,the combination of astragaloside Ⅳ and graphene oxide is more effective in the treatment of gastric ulcer in mice.