The Influence Of Different Treatments On The Root Surface Of Severe Periodontitis Teeth

Objective: This study aimed to evaluate the treatment of four different methods on root surfaces of teeth affected by severe periodontal disease,including the effect on surface morphology and adhesion and proliferation of Porphyromonas gingivalis（P.gingivalis）and human periodontal ligament cells（h PDLCs）on root surfaces.This study will provide a theoretical basis for clinical trials and clinical treatment of severe periodontitis.Methods: Teeth affected by severe periodontitis（n=100）were extracted and treated with scaling and root planning（SRP）,were prepared to slices.These slices were randomly divided into four groups: SRP group,Periocline group,EDTA group and Nd:YAG group.Scanning electron microscopy（SEM）and atom force microscope（AFM）were used to determine the surface morphology and the roughness of the root slices.The P.gingivalis and h PDLCs were cultured on the surfaces of root slices.The proliferation of P.gingivalis and h PDLCs was evaluated by cell count kit-8（CCK-8）.Results: The smear layer was almost completely removed in Periocline group and Nd:YAG group;whereas the obvious smear layer discovered in SRP group.The roughness of the root surface of Periocline group was the lowest while the Nd:YAG group was the highest.The amount of P.gingivalis adhered to the root surfaces of Periocline group and Nd:YAG group was significantly lower than other groups.However,after day 3,there were no statistical difference between the 4 groups.The attachment and proliferation of h PDLCs to the root surfaces showed a time dependent manner,and the number of attached cells was higher in Periocline group and Nd:YAG group.Conclusions: SRP combined with Periocline or Nd:YAG laser is more conducive to the reduction of the adhesion of P.gingivalis at the early stage and the attachment of and proliferation of h PDLCs.