Immunomodulatory Effects Of Oligodeoxynucleotide On Human Periodontal Ligament Cells Infected With Pg

Periodontitis is a chronic infectious disease of periodontal tissue caused by bacteria in dental plaque and other factors.It can lead to alveolar bone resorption and tooth loss,affect masticatory function and even affect the quality of life.At present,we control the development of disease inflammation through periodontal basic treatment,but it is difficult to completely and effectively restore lost alveolar bone tissue.In view of this clinical problem,promoting periodontal tissue regeneration has attracted much attention.We promote periodontal tissue repair by implanting growth factors,scaffolds and seed cells to remodel alveolar bone.This has become the key to periodontal clinical development.In recent years,it has been found that oligodeoxynucleotide(ODN)are involved in cellular immunity and bone regeneration,which is of great significance.In previous experiments,immunosuppressive ODN MT01 could promote osteogenic differentiation of human osteoblast-like cells MG63 in infected.In this study,12 different ODN sequences were used to stimulate the culture of human periodontal ligament cells.ODN that promotes proliferation and enhances ALP activity can be screened out.The selected ODN was used to stimulate healthy and infected human periodontal ligament stem cells.Cell cycle,apoptosis and expression of inflammatory cytokines were detected and compared.Method:1.HPDLCs are extracted and cultured in vitro.Porphyromonas gingivalis is cultured.2.The proliferation activity of hPDLCs stimulated by 12 ODN was detected by MTT assay.3.Alkaline phosphatase activity was detected by 12 ODN by diphenyldisodium phosphate microplate method.4.The cell cycle and apoptosis of healthy and infected hPDLCs stimulated by ODN were detected by flow cytometry.5.The expression of IL-1?,IL-6 and TNF-a in healthy and infected hPDLCs stimulated by ODN was detected by real-time quantitative PCR.Result:Compared with PBS group,ODN 7(YW002)promoted the proliferation activity and intracellular ALP activity of hPDLCs;ODN YW002 decreased the early and late apoptotic rate of hPDLCs in healthy and infected state,decreased the percentage of hPDLCs in G1 phase and increased the percentage of hPDLCs in S phase;ODN YW002 inhibited the expression of IL-1?,TNF-a and IL-6 in infected hPDLCs cells.Conclusion:1.Screening out two specific sequences of ODN,ODN YW002 and ODN MT01,respectively,can promote the proliferation of hPDLCs and promote osteogenic differentiation.2.ODN YW002 and ODN MT01 can shorten the G1 phase and enter the S phase of healthy and infected hPDLCs.3.ODN YW002 and ODN MT01 inhibit the apoptosis of healthy and infected hPDLCs.4.ODN YW002 can stably inhibit the expression of inflammatory factors in infected hPDLCs.