Facile,fast And Label-free Colorimetric Biosensor Based On Anti-aggregation Of Gold Nanoparticles For Determination Of Adenosine Triphosphate In Breast Cancer Cells

In this work,a facile,fast and label-free colorimetric biosensor for the detection of adenosine triphosphate(ATP)in T47D breast cancer cells is developed based on gold nanoparticles(AuNPs)as colorimetric probe and 4-mercaptophenylboronic acid(MPBA)as recognition element for ATP.MPBA has specific affinity for AuNPs through Au-S interaction and three boronic acid groups crosslink with the formation of boroxine ring,inducing the aggregation of AuNPs with a visible color change from red to blue.In the presence of ATP,the boronic acid group of MPBA preferentially binds to 2’,3’-hydroxyl groups of ATP to form stable borate ester,the MPBA-induced aggregation of AuNPs progressively decreased with an increase in the concentration of ATP,revealing an obvious blue-to-red color.The absorbance ratios(A520/A683)were linear with the ATP concentrations in the range of 8 to 100μM(R=0.9902)with a limit of detection to be 0.12 μM(3σ).The proposed colorimetric biosensor was successfully applied to the determination of ATP in T47D breast cancer cells and cultured cells with anticarcinogen.The concentration of ATP in T47D breast cancer cells was 51.30 ± 1.2 μmol/L with recovery between 99.4%to 102.2%(RSD≤4.2%).