Effect Of Adenosine Triphosphate Magnesium Chloride On Lung Adenocarcinoma Cell

Objective:To investigate the anticancer function and mechanism of adenosine triphosphate magnesium chloride (ATP-MgCl2) on lung adenocarcinoma cell.Methods:Human lung adenocarcinoma cell line(A549) was divided into four groups,(DATP-MgCl2 group:which was added with ATP-MgCl2 (0. 38mM) ;(2) ATP group:which was added with ATP (0. 38mM) ;(3) MgCl2 group:which was added with MgCl2 (0. 38mM);(4) control group:which was added with same dosage of normal saline. Each group was added with corresponding drug every day to the newly changed culture medium. During four days culture,the inhibiting rate of cancer cells was measured by cell counting and the change of exterior and interior cellular structure was observed by electron microscope. Telomerase activity of the cancer cell lines was measured by Telomeric Repeat Amplification Protocol (TRAP) assay.Results:The growth of cancer cells was inhibited markedly in ATP-MgCl2 group and ATP group (P<0. 01) ,lots of cells were killed ultimately. ATP-MgCl2 showed higher growth inhibition rate and cytotoxic activity than ATP in the first two days (P<0. 05) . In MgCl2 group,the growth of cancer cells was unaffected. With electron microscope,we observed that in ATP group the microvilli of cancer cells were shortened and reduced markedly,the nucleoli became annular. In the group of ATP-MgCl2,the microvilli of cancer cells were shortened significantly and the number reduced significantly or even disappeared,the surface of cells became velvet,nucleoli became annular too. Positivities for telomerase activity in ATP-MgCl2 and ATP group were significantly lower than both in the control group and MgCl2 group (P<0. 01),but there was no marked difference between ATP-MgCl2 and ATP group (P>0. 05) .Conclusion:According to these results,we think ATP-MgCl2 not only induces differentiation,but also shows a marked inhibitory and cytotoxic activity in a human adenocarcinoma cell line (A549). Its anticancer mechanism likes ATP's,is relevant to inhibiting the activity of telomerase in cancer cell. But because MgCl2 can form an active compound with ATP,which increases the anticancer function of ATP,ATP-MgCl2 has a preferable clinical value.