The Study Onscreening Of Effective Fermentation Bacteriafrom Mirabilitum Praeparatumand And Chemicalconstituents Of Effective Parts For Antibiosi In Mirabilitum Praeparatumand

Mirabilitum Praeparatum,also known as watermelon nitrate or watermelon frost,is Cucurbitaceae plant watermelon[Citrullus Lanatus(Thunb.)Matsumu.et Nakai]processing with mirabilite and made of cream.The taste of cold salty,into the spleen.According to the theory of traditional Chinese medicine(TCM)watermelon frost Qingre Xiehuo,detumescence and acesodyne effect source on watermelon and Glauber’s salt.However,our research group found that watermelon cream frosting processing method,in fact,is a fermentation process,some halophilic microorganisms involved in the fermentation of watermelon frost,secondary metabolites with antimicrobial activity,possibly as a material basis for the efficacy of watermelon frost.Research Group basing on the theory of traditional Chinese medicine,combined with method of biotechnology,microbial immunological methods and traditional Chinese medicine chemical methods,optimization of fermentation technology for watermelon frost,and determined the antibacterial effective parts,isolation and identification of microbes and identification and effective strain screening and antibacterial effective parts of the chemical components of the separation and identification of compounds and other aspects of the inquiry.The main research contents are as follows:In order to prove the effectiveness of the antibacterial extract of watermelon frost,and its effect in mirabilite on antibacterial effect,Firstly in vivo antibacterial experiments that watermelon frost methanol extracts in vivo has good inhibitory effect on Gram positive and gram negative bacteria,Glauber’s salt no antibacterial effect.In order to meet the further screening of effective strains for fermentation and purebred fermentation technology aspects of,this experiment in watermelon frost in the fermentation process of fermentation broth were sampled samples of each stage of strain isolation,purification and identification,5 strains were obtained,were identified by the morphology and ITS sequence,identified Cyberlindnera jadinii),Penicilium commune,Rhodosporidium diobovatum,Rhizopus stolonifer,Penicilium rubens,5 strains of bacteria in watermelon frost are isolated for the first time,respectively.For further screening effective fermentation strains,and to explore the role of Glauber’s salt in watermelon frost processing process,this paper by pure breed fermentation,the use of pure breed fermentation extracts in vitro antibacterial test.In the end,we selected two strains showed antimicrobial effect of strain commune penicilium and penicilium Rubens,respectively.Found in the comparison of different components of the fermentation medium,extract medium containing only 15%extraction mirabilite has antibacterial effect.Prompt mirabilite although there is no direct antimicrobial effect,but in the fermentation process of watermelon frost,Glauber’s salt to an important role.In vivo antibacterial experiments were made on the basis of the antibacterial effect of the methanol extract of watermelon frost.6 compounds were separated and identified by the silica gel chromatography,ODS column chromatography and high performance liquid chromate graphy,and compounds were separated and identified by 1H-NMR spectrum and 13 C-NMR spectrum.Respectively as(1),(1 S,3 S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid,(2),phenylmethyl-6-O-β-D-xylopy ranosyl(1→6)-β-D-glucopyranoside(3),methyl 2[2-formyl-5-(methoxymethyl)-1H-pyrrol-1-yl]propanoate(4),methyl 3-(p-hydroxyphenyl)propionate、(5),4-(2-formyl-5-methoxy methylpyrrol-1-yl)butyric acid methyl ester(6),1,3dimethyl-2,3,4,9-tetrahydro-lH-pyrido[3,4-b]indole.The 2,3,4,5,6 compounds were first isolated from watermelon frost.The through pharmacodynamic experiments identified the effective part of watermelon frost for the methanol extract and the the studies on the chemical constituents,further up the watermelon frost research and further clarify the material basis for the efficacy of watermelon frost.In addition,to microbial metabolites for the starting point to explore the watermelon frost of processing the basic principle opens up the processing research ideas,but also for the formulation of watermelon frost pharmacodynamic material basis research,processing technology and quality standard has an important role in promoting,and to reveal the connotation of traditional Chinese medicine fermentation is of great significance.