Identification Of RNA Transcripts And Construction Of RNA Network In Chronic Obstructive Pulmonary Disease

Purpose Chronic obstructive pulmonary disease（COPD）is one of the leading causes of death worldwide and a major chronic disease,highly prevalent in the aging population exposed to tobacco smoke and airborne pollutants,At this time,early disease predictors and targeted therapies are particularly important.Abnormal expression of non-coding RNAs in COPD has been identified.however,few studies have systematically analyzed the interactions between different RNA transcripts in COPD.Therefore,RNA regulatory networks such as lnc RNA-mRNA,circ RNA-mi RNA-mRNA interaction networks can facilitate our understanding of the mechanism of RNA dysregulation in COPD.Therefore,we examined the expression of RNA transcripts in RNA-Seq of COPD patients and further constructed possible RNA regulatory networks.Methods All fresh peripheral blood samples of three patients with COPD and three healthy controls were collected and examined for mRNA,mi RNA,lnc RNA,and circ RNA expression,and then the samples were expanded for q RT-PCR validation of some of the results.We also examined mRNA expression to identify relevant biological pathways associated with COPD.lnc RNA-mRNA coexpression network and circ RNAmi RNA-mRNA network in COPD were constructed.Results In this study,we have comprehensively identified and analyzed the dysregulated mRNAs,lnc RNAs,mi RNAs,and circ RNAs in peripheral blood of COPD patients with RNA-Seq.282 mRNAs,146 lnc RNAs,85 mi RNAs,and 81 circ RNAs were differentially expressed.GSEA analysis showed that these dysregulated RNAs correlate with several critical biological processes such as “nc RNA metabolic process”,“nc RNA processing”,“Ribosome biogenesis”,“r RNAs metabolic process”,“t RNA metabolic process” and “t RNA processing”,which might be participating in the progression of COPD.RT-q PCR with more clinical COPD samples were used for the validation of some dysregulated RNAs,and the results were in high accordance with the RNA sequencing.Given the putative regulatory function of lnc RNAs and circ RNAs,we have constructed the co-expression network between lnc RNA and mRNA and the competing endogenous RNA（ce RNA）network of differential expression circ RNAmi RNA-mRNA in COPD.Conclusions In this study,we have identified and analyzed the differentially expressed mRNAs,lnc RNAs,mi RNAs,and circ RNAs,providing a systematic perspective on the involvement of the dysregulated RNA in the pathogenesis of COPD.We have also constructed the lnc RNA-mRNA co-expression network,and circ RNA-mi RNA-mRNA network in COPD.This study provides valuable insights for further analysis of the mechanism of RNA action in COPD.